Biogeography of Cryoconite Bacterial Communities Across Continents

The geographic distribution patterns of microorganisms and their underlying mechanisms are central topics in microbiology, crucial for understanding ecosystem functioning and predicting responses to global change. Cryoconite absorbs solar radiation to form cryoconite holes, and because it lies within these relatively deep holes, it faces limited interference from surrounding ecosystems, often being seen as a fairly enclosed environment. Moreover, it plays a dominant role in the biogeochemical cycling of key elements such as carbon and nitrogen, making it an ideal model for studying large-scale microbial biogeography. In this study, we analyzed bacterial communities in cryoconite across a transcontinental scale of glaciers to elucidate their biogeographical distribution and community assembly processes. The cryoconite bacterial communities were predominantly composed of Proteobacteria, Cyanobacteria, Bacteroidota, and Actinobacteriota, with significant differences in species composition across geographical locations. Bacterial diversity was jointly driven by geographical and anthropogenic factors: species richness exhibited a hump-shaped relationship with latitude and was significantly positively correlated with the Human Development Index (HDI). The significant positive correlation may stem from nutrient input and microbial dispersal driven by high-HDI regions' industrial, agricultural, and human activities. Beta diversity demonstrated a distance-decay pattern along spatial gradients such as latitude and geographical distance. Analysis of community assembly mechanisms revealed that stochastic processes predominated across continents, with a notable scale dependence: as the spatial scale increased, the role of deterministic processes (heterogeneous selection) decreased, while stochastic processes (dispersal limitation) strengthened and became the dominant force. By integrating geographical, climatic, and anthropogenic factors into a unified framework, this study enhances the understanding of the spatial-scale-driven mechanisms shaping cryoconite bacterial biogeography and emphasizes the need to prioritize anthropogenic influences to predict the trajectory of cryosphere ecosystem evolution under global change.