Related Experiment Video
Updated: Jan 29, 2026

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
Published on: March 22, 2020
Highly Sensitive Detection of Chymotrypsin Using Gold Nanoclusters with Peptide Sensors
Siyuan Zhou1, Cheng Liu2, Haixia Shi3
1Institute of Cancer Research, Affiliated Hospital of Jiangsu University, Zhenjiang 212013, China.
None:
Pancreatic function tests are used to determine the presence of chronic pancreatitis, particularly in the early stage of the disease. Chymotrypsin is an indicator of pancreatic function and is thus related to pancreatic diseases. However, these methods often require specific equipment and cannot always meet on-site analysis requirements. Consequently, a highly sensitive detection method needs to be developed. This research employed graphene oxide modified with NHS sensors and peptides (RRHFFGC: Arginine-Arginine-Histidine-Phenylalanine-Phenylalanine-Glycine-Cysteine) tagged with gold nanoclusters (Au NCs) for the detection of chymotrypsin. The N-Hydroxysuccinimide-(Polyethylene Glycol)4-Dibenzocyclooctyne (NHS-PEG4-DBCO) and graphene oxide (GO)-N3 click reaction yielded GO-NHS material, appropriate for fluorescence quenching. The peptide chain was accurately broken with the introduction of chymotrypsin, and the Au NCs were situated far from the GO-NHS surface. The detection limit was 2.014 pg/mL. The results showed that the detection method had high sensitivity in comparison with the previous studies. This method is relevant to real samples due to its potential efficacy. Therefore, it is a promising method in the biomedical field.
Related Concept Videos
Peptide Bonds
Sensitivity, Specificity, and Predicted Value
Sensitivity is the...
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Urine Studies II: Urine Culture and Sensitivity Test
Local Anesthetics: Differential Sensitivity of Nerve Fibers

