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Peritoneal anaphylaxis in the rat after sensitization with mouse antiserum
International Archives of Allergy and Applied Immunology
|January 1, 1978
Summary
Passive peritoneal anaphylaxis in rats demonstrated IgE-mediated characteristics. Disodium cromoglycate (DSCG) and sodium nivimedone inhibited histamine and slow-reacting substance of anaphylaxis (SRS-A) release, with nivimedone being more potent.
Area of Science:
- Immunology
- Pharmacology
Background:
- Passive peritoneal anaphylaxis (PPA) is a model to study allergic reactions.
- Understanding the mediators and inhibitors of anaphylaxis is crucial for developing treatments.
Purpose of the Study:
- To characterize the PPA reaction in rats as IgE-mediated.
- To evaluate the inhibitory effects of disodium cromoglycate (DSCG) and sodium nivimedone on anaphylaxis mediators.
Main Methods:
- Rats were sensitized with mouse antiserum to induce PPA.
- Rats were pretreated with DSCG or sodium nivimedone.
- Histamine and slow-reacting substance of anaphylaxis (SRS-A) levels in peritoneal fluid were measured after antigen challenge.
Main Results:
- The PPA reaction exhibited heat-labile serum properties, indicative of IgE mediation.
- Both DSCG and sodium nivimedone significantly inhibited histamine and SRS-A release.
- Sodium nivimedone demonstrated greater potency than DSCG in inhibiting histamine release.
- Peak mediator concentrations were observed within 2 minutes post-challenge, returning to baseline by 20-30 minutes.
Conclusions:
- The PPA model in rats effectively mimics IgE-mediated anaphylaxis.
- DSCG and sodium nivimedone are effective inhibitors of anaphylactic mediator release.
- Sodium nivimedone shows superior efficacy over DSCG in suppressing histamine release during anaphylaxis.