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Updated: Jan 29, 2026

Laboratory Rearing of Stable Flies and Other Muscoid Diptera
Published on: August 3, 2018
Effect of Rearing Substrate on Nutritional Composition, Growth Performance and Multi-Omics Characteristics of Black
Kun Liu1, Guangming Zhang2, Yuting Li3
1Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
None:
To address global protein shortages, black soldier fly (Hermetia illucens L.) larvae (BSFL) can be used as a sustainable feed alternative, with nutritional quality dependent on rearing substrates. This study compared quail feed (QF), food waste (FW), and quail manure (QM) for BSFL cultivation. Larvae raised on FW and QM had higher crude protein content (47.5-48.3%) than the QF group, while QF-reared larvae contained more fat (33.2%) than the other groups. QM led to larvae with the highest mineral content, and larvae from the FW group showed elevated calcium levels (5.6%). The FW-reared larvae also demonstrated superior growth, with a yield of 186.3 g/kg and a dry-matter conversion rate of 13.7%. Multi-omics analyses indicated substrate-specific differences in gut microbiota and metabolic pathways. FW promoted beneficial bacteria such as Lactobacillus, enhancing nutrient assimilation, while QF and QM upregulated lipid and mineral processing. For sustainable protein production through BSFL rearing, FW was the most suitable substrate among the three tested, offering a balanced combination of high larval protein content and efficient growth.
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