Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Asthma-I: Introduction01:29

Asthma-I: Introduction

3.5K
Asthma is a chronic respiratory ailment that requires careful management due to its varying symptoms and influencing factors. It is characterized by airway inflammation, bronchial hyperresponsiveness, and reversible airflow obstruction, leading to symptoms like wheezing, shortness of breath, chest tightness, and coughing. The symptom frequency and intensity may vary considerably over time. It is also linked to immune system responses to allergens and irritants, highlighting the complex...
3.5K
Asthma-III: Symptoms and Complications01:24

Asthma-III: Symptoms and Complications

3.3K
Asthma, a common chronic respiratory condition, is classified considering the frequency and severity of symptoms alongside lung function impairment. Understanding this classification is essential for appropriate treatment and management. Here's a detailed look at the classification of asthma and its clinical features and complications:
Classification of Asthma
3.3K
Asthma-IV: Diagnostic and Management01:30

Asthma-IV: Diagnostic and Management

3.1K
The diagnosis and management of asthma are comprehensive, encompassing clinical assessments, lung function tests, and pharmacological interventions. Here's an overview:
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
3.1K
Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

1.3K
Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
1.3K
Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

4.3K
Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
4.3K
Asthma-IV: Nursing Management01:30

Asthma-IV: Nursing Management

3.8K
The nursing management of asthma is a comprehensive approach that relies heavily on the expertise and dedication of healthcare professionals. It involves thorough assessment, accurate diagnosis, strategic planning, effective implementation, and diligent evaluation. By meticulously following this step-by-step process, healthcare professionals play a crucial role in providing the best possible care and treatment for patients with asthma, enhancing their overall health and well-being.
First, in...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cockroach sensitization and its hidden links to mite and food allergens.

Scientific reports·2026
Same author

Exacerbations and Lung Function in Polish Patients with Chronic Obstructive Pulmonary Disease Treated with ICS/LABA: 2-Year Prospective, Observational Study.

Journal of clinical medicine·2025
Same author

Vitamin D and Atopic Dermatitis-A Mere Correlation or a Real Supportive Treatment Option?

Nutrients·2025
Same author

Drugs Used in the Treatment of Viral Infections for the Prevention of Airway Remodeling in Asthma.

Mediators of inflammation·2025
Same author

The therapeutic potential of vitamin D supplementation in asthma.

Pharmacological reports : PR·2025
Same author

The potential effect of gut microbiota on the secretion of selected cytokines by human cells in vitro.

Scientific reports·2025

Related Experiment Video

Updated: Jan 29, 2026

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Published on: February 15, 2019

14.8K

Gut Microbiota Modulation in Asthma-An In Vitro Study.

Paulina Kleniewska1, Paulina Natalia Kopa-Stojak1, Rafał Pawliczak1

  • 1Department of Immunopathology, Faculty of Medicine, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland.

Pharmaceuticals (Basel, Switzerland)
|January 28, 2026
PubMed
Summary

Selected bacterial lysates, including Clostridium perfringens (CP) and Parabacteroides distasonis (PD), reduced key immune signaling molecules like IL-17 and INF-γ in human cells. These effects were dose-dependent but may not significantly impact overall immune status.

Keywords:
Bacteroides vulgatusClostridium perfringensParabacteroides distasonisRuminococcus albusasthmaintestinal microflora

More Related Videos

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
06:58

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

7.5K
Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation
08:34

Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation

Published on: July 27, 2022

1.2K

Related Experiment Videos

Last Updated: Jan 29, 2026

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Published on: February 15, 2019

14.8K
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
06:58

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

7.5K
Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation
08:34

Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation

Published on: July 27, 2022

1.2K

Area of Science:

  • Immunology
  • Microbiology
  • Cell Biology

Background:

  • The gut microbiome plays a crucial role in immune system regulation.
  • Bacterial components can modulate host immune responses.
  • Understanding specific bacterial lysate effects on immune cells is vital.

Purpose of the Study:

  • To investigate the immunomodulatory effects of lysates from four bacterial species: Bacteroides vulgatus (BV), Clostridium perfringens (CP), Parabacteroides distasonis (PD), and Ruminococcus albus (RA).
  • To determine the impact of these bacterial lysates on the secretion of IL-17, INF-γ, IL-2, and TGF-β1 by human HT-29 cells, peripheral blood mononuclear cells (PBMCs), and monocytes (MON).
  • To assess dose-dependent responses of immune cells to bacterial lysates.

Main Methods:

  • Incubation of human HT-29 cells, PBMCs, and MON with varying doses (100 µg and 400 µg) of bacterial lysates (BV, CP, PD, RA).
  • Measurement of cytokine secretion (IL-17, INF-γ, IL-2, TGF-β1) using appropriate assays.
  • Statistical analysis to compare cytokine levels between treated groups and controls.

Main Results:

  • CP, RA, and PD lysates significantly reduced IL-17 secretion by HT-29 cells in a dose-dependent manner.
  • BV and PD lysates significantly decreased IL-17 secretion by PBMCs and MON.
  • PD and CP lysates lowered INF-γ secretion by HT-29 cells, while CP and BV lysates reduced INF-γ in MON and PBMCs, respectively.

Conclusions:

  • Selected bacterial lysates, particularly CP and PD, can significantly lower the secretion of IL-17 and INF-γ by human immune cells (HT-29, PBMCs, MON).
  • The observed immunomodulatory effects are dose-dependent.
  • The magnitude of cytokine reduction in this study suggests a limited impact on overall systemic immune status.