Sorbitol-Stabilized Silicon Formulation Improve Root Traits and Antioxidant Response in Drought-Stressed Soybean

Silicon (Si) plays a critical role in regulating plant physiological processes, particularly through its influence on non-enzymatic antioxidant systems and amino acid metabolism. This study aims to assess soybean performance in response to both soil and foliar Si applications under well-watered and drought conditions, with the goal of enhancing Si accumulation in plant tissues and potentially strengthening the crop's physiological responses to water deficit stress. This is especially pertinent given that the mechanisms underlying Si fertilization and its contribution to drought tolerance in soybean remain poorly understood. Greenhouse experiments were conducted using a 3 × 2 factorial design. The factors were: (i) three foliar Si treatments: control (no Si), potassium silicate (SiK; 128 g L^-1 Si, 126.5 g L^-1 K₂O, pH 12.0), and sorbitol-stabilized potassium silicate (SiKe; 107 g L^-1 Si, 28.4 g L^-1 K₂O, 100 mL L^-1 sorbitol, pH 11.8); and (ii) two soil water levels: well-watered (80% field capacity) and water-restricted (40% field capacity), the latter simulating tropical dry spells. Silicon was applied to the soil via irrigation and to the leaves via foliar spraying prior to the onset water restriction. All Si solutions were adjusted to pH 7.0 with 1 M HCl immediately before application. Potassium (K) levels were standardized across treatments through supplementary applications of KCl to both soil and foliage. Biometric and physiological parameters were subsequently measured. Sorbitol-stabilized Si enhanced Si accumulation in soybean tissues and improved plant resilience under both well-watered and drought conditions by promoting key physiological traits, including increased levels of daidzein and ascorbic acid levels, along with reduced amino acid concentrations. It also improved biometric parameters such as leaf area, root development, and number of pods per plant. These findings further support the role of Si as a beneficial element in enhancing stress tolerance and contributing to sustainable agricultural practices.