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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Published on: May 27, 2016
The CiWOX13-CiWOX14 Complex Regulates CiBGLU21 to Promote Graft Union Formation by Modulating Cell Wall Synthesis in
Piyu Ji1,2, Wanchun Li1,2, Liangye Huang1,2
1State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
Abstract:
Grafting is an important method for pecans, while the molecular mechanisms underlying graft union formation still need in-depth analysis. In the current investigation, we identified 22 BGLU genes in Carya illinoinensis (pecan) and demonstrated that CiBGLU21, a β-glucosidase-encoding gene, plays an important positive role in graft healing. The overexpression of CiBGLU21 enhanced graft survival rates and accelerated tissue regeneration, while biochemical assays confirmed its role in cell wall reinforcement and sugar metabolism. Additionally, we identified that CiWOX13 formed heterodimers with CiWOX14 to directly and synergistically activate the transcription of CiBGLU21. The current investigation revealed a CiWOX13/14-CiBGLU21 module as an important modulator of graft union formation, offering insights into improving grafting efficiency in perennial crops and advancing the understanding of cell wall dynamics during tissue regeneration.
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