Related Experiment Video

Updated: Jan 29, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.8K

Genomic Characterization of a Mycoplasma ovipneumoniae Strain from Hu Sheep in Inner Mongolia, China

Lingli Dai1, Na Wang1, Fan Zhang1

  • 1Veterinary Research Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China.

Veterinary Sciences
|January 28, 2026
PubMed

Insights

A novel Mycoplasma ovipneumoniae strain from Inner Mongolia was sequenced, revealing unique genetic traits and potential virulence factors. This genomic data aids in understanding sheep respiratory disease and developing targeted control strategies.

Area of Science:

  • Veterinary Microbiology
  • Genomics
  • Animal Pathology

Background:

  • Mycoplasma ovipneumoniae is a significant pathogen causing respiratory disease in sheep, leading to economic losses.
  • Uncommon pathological manifestations, such as pulmonary adenomatous-like lesions, highlight the need for further investigation into M. ovipneumoniae strains.

Purpose of the Study:

  • To characterize a novel Mycoplasma ovipneumoniae strain (IM-DMQ) isolated from a Hu sheep in Inner Mongolia.
  • To perform whole-genome sequencing and comparative genomic analysis of the IM-DMQ strain.
  • To investigate the genetic basis of its pathogenicity and potential virulence factors.

Main Methods:

  • Isolation and identification of Mycoplasma ovipneumoniae strain IM-DMQ from affected sheep.
  • Whole-genome sequencing using a hybrid Nanopore and Illumina approach.
  • Comparative genomic analysis with global M. ovipneumoniae strains and microbial community profiling.

Main Results:

  • The complete genome of IM-DMQ was sequenced (1,039,804 bp, 29.15% GC content, 1529 genes).
  • IM-DMQ shares high genomic similarity with the Chinese strain NXNK2203 (ANI: 98.3%) but possesses unique genes and structural variations.
  • Co-infection with other bacterial pathogens was identified in the original lung tissue.

Conclusions:

  • This study presents the first complete genome of an M. ovipneumoniae strain from Inner Mongolia associated with unusual pulmonary pathology.
  • The genomic insights contribute to understanding the genetic diversity and evolution of M. ovipneumoniae.
  • Findings support the development of targeted diagnostics and vaccines for controlling sheep respiratory disease in the region.
Keywords:
Hu sheepMycoplasma ovipneumoniaecomparative genomicsgenome sequencingpulmonary adenomatous-like lesions

More Related Videos

Optimized PCR-based Detection of Mycoplasma06:01

Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

55.0K
Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae06:04

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae

Published on: February 24, 2023

2.9K

Related Concept Videos

Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
7.4K
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.4K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.1K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.1K
Thermal Strain01:19

Thermal Strain

Thermal strain is a concept that arises when we consider how temperature changes affect structures. Unlike the conventional assumption that structures remain constant under load, real-world scenarios often involve temperature fluctuations that can significantly impact these structures. Consider a homogeneous rod with a uniform cross-section resting freely on a flat horizontal surface. If the rod's temperature increases, the rod elongates. This elongation is proportional to the temperature...
2.8K
Shearing Strain01:20

Shearing Strain

The shearing strain represents a cubic element's angular change when subjected to shearing stress. This type of stress can transform a cube into an oblique parallelepiped without influencing normal strains. The cubic element experiences a significant transformation when exposed solely to shearing stress. Its shape alters from a perfect cube into a rhomboid, clearly demonstrating the effect of shearing strain. The degree of this strain is considered positive if it reduces the angle between the...
1.4K