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Related Experiment Video

Updated: Jan 29, 2026

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
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Anti-HIV-1 Activity of the Integrase Strand Transfer Inhibitor ACC017.

Meng-Di Ma1,2, Rong-Hua Luo1, Chun-Yan Li1,3

  • 1State Key Laboratory of Genetic Evolution & Animal Models, Key Laboratory of Bioactive Peptides of Yunnan Province, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.

Viruses
|January 28, 2026
PubMed
Summary

ACC017 is a new HIV-1 integrase strand transfer inhibitor (INSTI) showing potent antiviral activity. It is effective against drug-resistant HIV strains and demonstrates a favorable resistance profile, making it a promising therapeutic candidate.

Keywords:
ACC017HIV-1INSTIsantiviral activitypharmacodynamics

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Area of Science:

  • Virology
  • Medicinal Chemistry
  • Drug Discovery

Background:

  • HIV-1 integrase strand transfer inhibitors (INSTIs) are crucial in antiretroviral therapy.
  • Emergence of drug-resistant mutations necessitates novel therapeutic agents.

Purpose of the Study:

  • To introduce ACC017 as a novel INSTI candidate.
  • To evaluate the antiviral activity and resistance profile of ACC017.

Main Methods:

  • In vitro antiviral activity assays against HIV-1 strains.
  • Mechanism of action studies to assess integrase inhibition.
  • In vitro resistance selection studies.

Main Results:

  • ACC017 exhibited potent activity against HIV-1IIIB (EC50 = 0.59 nM) and drug-resistant strains (EC50 range: 0.11–9.12 nM).
  • Inhibition of HIV-1 integrase enzyme with IC50 = 9.19 nM.
  • Resistance selection yielded D232N and R263K mutations, avoiding common resistance mutations.

Conclusions:

  • ACC017 demonstrates potent antiviral activity and a favorable resistance profile.
  • Synergistic interactions with other antiretroviral drugs suggest therapeutic potential.
  • ACC017 is a promising candidate for further development in HIV treatment.