Related Experiment Video

Updated: Jan 29, 2026

A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants
14:56

A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants

Published on: February 8, 2012

13.4K

Tetraspanin CD9 Is a Positive Regulator of Filovirus Egress

Loveleena K Anand1, Marija A Djurkovic2, Ariel Shepley-McTaggart1

  • 1Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, USA.

Viruses
|January 28, 2026
PubMed

Insights

Tetraspanin CD9 facilitates the efficient egress of Ebola (EBOV) and Marburg (MARV) viruses. This finding identifies CD9 as a potential target for developing new antiviral therapies against filoviruses.

Area of Science:

  • Virology
  • Cell Biology
  • Immunology

Background:

  • Filoviruses, such as Ebola (EBOV) and Marburg (MARV) viruses, are zoonotic pathogens causing severe hemorrhagic fever with high mortality rates.
  • Viral egress and spread are orchestrated by the matrix protein VP40 and modulated by host-cell interactions.

Purpose of the Study:

  • To investigate the role of tetraspanin CD9 in the egress of EBOV and MARV.
  • To identify CD9 as a potential therapeutic target for filovirus infections.

Main Methods:

  • Utilized CD9-knockdown (KD) cells and wild-type (WT) cells to assess viral egress.
  • Quantified the egress of virus-like particles (VLPs) and live filoviruses.
  • Assessed the rescue effect of exogenous CD9 expression on viral egress.

Main Results:

  • A significant reduction in viral egress was observed in CD9-KD cells compared to WT cells.
  • Exogenous expression of CD9 restored VP40 VLP egress in CD9-KD cells to levels near those in WT cells.
  • Tetraspanin CD9 acts as a positive regulator of filovirus egress.

Conclusions:

  • Tetraspanin CD9 plays a crucial role in facilitating efficient filovirus egress.
  • CD9 represents a potential novel target for antiviral strategies aimed at inhibiting the late stages of the filovirus lifecycle.
Keywords:
EbolaMarburgVLP egressfilovirustetraspanin CD9virus–host interaction

More Related Videos

Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors08:39

Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors

Published on: January 7, 2019

9.1K
Characterizing Extracellular Vesicles from Biological Fluids05:07

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

860

Related Concept Videos

Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
136.1K
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
6.9K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.8K
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
7.1K
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.7K
GTPases and their Regulation02:14

GTPases and their Regulation

Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.8K