[Recommendations for the Standardization of Forensic Microbiological Analysis]

With advances in microbiome research and the continuous advancement of detection technologies, the application of microorganisms in forensic medicine has become increasingly widespread, covering areas such as individual identification, body fluid source inference, biogeographical analysis, postmortem interval estimation, and determination of the cause and location of death. However, due to the lack of a comprehensive standardized system, batch effects and inter-laboratory differences have led to low reproducibility of analysis results. This problem is particularly evident in low-quality forensic samples, which compromise the reliability and evidential value of forensic microbiological analyses. Therefore, based on domestic and international research progress and practical experience, this paper systematically summarizes and discusses the standardization of forensic microbiological analysis, aiming to improve the reliability of results and promote the standardization of forensic microbiological analysis.