Related Experiment Video
Updated: Jan 29, 2026

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
Published on: June 13, 2019
Unveiling the hidden: diagnosing isolated myeloid sarcoma through ascitic fluid analysis
Zhengyu Zhou1, Zhanyi Yue1, Landi Zhang1
1Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical University, Shanghai, China.
Introduction:
Isolated myeloid sarcoma, defined as an extramedullary manifestation of acute myeloid leukemia with essentially normal bone marrow, is frequently misdiagnosed as other disorders due to its nonspecific presentation.
Methods:
A 31-year-old woman presented at Shanghai Changhai hospital with abdominal symptoms without obvious inducement.
Results:
Computed tomography scans revealed circumferential wall thickening of the terminal ileum with luminal narrowing, accompanied by moderate ascites. Primitive myeloid cells were identified in ascitic fluid through cytologic analysis. Flow cytometric analysis of the ascitic fluid showed an immunophenotypic profile consistent with myeloid blasts. Molecular analysis of the ascitic fluid identified the presence of CBFβ::MYH11 fusion genes. The bone marrow aspiration, biopsy, and fluorescence in situ hybridization test revealed normocellular marrow without abnormalities. The final diagnosis was isolated myeloid sarcoma.
Discussion:
Cytologic, flow cytometric, and molecular analyses of ascitic fluid can provide critical diagnostic evidence for myeloid sarcoma in cases where abdominal effusion is associated with myeloid sarcoma involvement.
More Related Videos
Related Concept Videos
Diagnosing Acidosis and Alkalosis
First, the pH level is assessed to determine whether the blood pH is normal (7.35–7.45), low (acidosis), or high (alkalosis).
Next, the PCO2 and...
Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
The Fluid Mosaic Model
Differentiation of Common Myeloid Progenitor Cells
Fluid Pressure
According to Pascal's law, a fluid at rest will generate equal pressure in all directions. This pressure is measured as a force per unit area, and its magnitude depends on the fluid's specific...
Accelerating Fluids
The motion of the liquid within this infinitesimal cylinder is considered to obtain the pressure difference. Three vertical forces act on this liquid:

