Tooth-Bone Integrated Organoids via Bioactive Glass Mediated Dual Interface Bonding and Rapid Osteogenesis.

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Oral and Maxillofacial Surgery

Background:

• Maxillofacial bone defects significantly impact oral function and aesthetics.
• Current treatments like autologous bone grafting involve high surgical trauma and lengthy recovery.

Purpose of the Study:

• To develop a tooth-bone integrated organoid (TBO) strategy for simultaneous in vitro reconstruction of bone and dental implant structures.
• To achieve dual restoration of structure and function for maxillofacial defects post-implantation.

Main Methods:

• Utilized bioactive glass (BG) as a multifunctional bonding agent.
• BG facilitated adhesion between BG callus organoids and BG-Si3N4 implants via dual-interface bonding.
• BG stimulated callus organoid hypertrophy and ossification, enhancing osteogenesis and osseointegration.

Main Results:

• Achieved effective adhesion between organoids and implants, promoting bone regeneration.
• Accelerated endochondral ossification and mineralization of callus organoids.
• Enhanced osteogenic potential and osseointegration of dental implants through bioactive ion release.

Conclusions:

• The TBO strategy offers a promising approach for repairing large maxillofacial bone defects.
• This method synchronously reconstructs bone and dental implants, restoring both structure and function.
• Presents a clinically translatable pathway for integrated maxillofacial regeneration.