Synergistic Intra- and Inter-Nanozyme Electron Transfer through Interfacial Assembly for Enhanced Multi-Enzyme...

Prussian Blue composite nanozymes (PB C-NZs) have been extensively employed in various biomedical applications. However, due to the complexity of its multi-enzyme activities and structural components, designing material composition and processes, optimizing specific enzymatic properties of materials, and exploring the scientific mechanisms involved remain important challenges. Given the equal importance of electron transfer optimization and intrinsic redox properties, we propose an interfacial assembly strategy that leverages electron transfer and energy band structure, allowing for the synergistic interactions between internal and interfacial electrons of the nanocomposites (MoS₂/PB) and thereby enhancing the enzyme-like activity. In the MoS₂/PB system, a distinct inter-nanozyme electron transfer is operative, facilitating directional electron transport from MoS₂ to PB, thereby enhancing catalytic activity. Concurrently, band modulation effects induced by the interaction between MoS₂ and PB effectively enhance the reductase-like catalytic activity. Notably, the expression of multiple enzyme activities can be enriched through band regulation. The comprehensive enzymatic activity tests demonstrated that MoS₂/PB exhibits enhanced multiple-enzyme activities, including catalase, peroxidase, superoxide dismutase, glutathione peroxidase, S-nitrosoglutathione reductase, and nitrite reductase. This study introduces a novel design concept for composite nanozymes based on electron transfer modulation, providing valuable insights and guidance for the development of high-performance nanozymes.