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Updated: Jan 29, 2026

Production of Haploid Zebrafish Embryos by In Vitro Fertilization
Published on: July 14, 2014
Simple and Efficient In Vitro Fertilization and Embryo Transfer in Mice
Fengjun Lv1, Shuangting Lang1, Ximing Qin1
1School of Life Sciences and Medical Engineering, Anhui University, Hefei, Anhui Province, China.
Abstract:
Mice, as one of the most important model organisms in life science research, rely heavily on assisted reproductive technologies, particularly in vitro fertilization (IVF) and embryo transfer (ET), which serve as indispensable platforms for genetic modification, strain preservation and expansion, as well as studies in reproductive biology. This article provides a detailed description of an optimized and validated experimental system encompassing the entire workflow from superovulation, sperm collection and capacitation, in vitro fertilization, embryo culture, and vasectomy of male mice, to embryo transfer and related auxiliary procedures. © 2026 Wiley Periodicals LLC. Basic Protocol 1: IVF in mice Basic Protocol 2: Oviduct ET in mice Support Protocol: Preparation of vasectomized males and pseudopregnant females.
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