Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

17.6K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
17.6K
Emerging Adulthood01:27

Emerging Adulthood

676
Jeffrey Arnett's concept of emerging adulthood offers a framework to understand the unique developmental stage between adolescence and full-fledged adulthood, generally from ages 18 to 25. This period is marked by extensive exploration and shifts in identity, relationships, and career choices, a process known in psychology as role experimentation. Emerging adulthood reflects the evolving cultural expectations surrounding adulthood and the dynamic process of personal transformation during...
676
Enzymes02:34

Enzymes

94.6K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
94.6K
Enzyme Kinetics01:19

Enzyme Kinetics

104.0K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
104.0K
Introduction Cardiac Emergencies01:30

Introduction Cardiac Emergencies

337
Cardiac emergencies are critical situations involving the heart that require immediate medical intervention to prevent severe complications or death. These emergencies often arise from underlying heart conditions that impair the heart's ability to function correctly.Types of Cardiac EmergenciesThe most common types of cardiac emergencies include Acute Coronary Syndrome (ACS), myocardial infarction (MI), cardiac arrest, and heart failure.Acute Coronary Syndrome (ACS)Acute Coronary Syndrome (ACS)...
337
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.1K
Overview
80.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-channel optogenetics in yeast for multiplexed light-based control of cellular processes and pathways.

Nature communications·2026
Same author

The quality and reliability of endometrial cancer-related videos on social short video platforms: a cross-sectional study.

BMC women's health·2026
Same author

Microbiota-metabolome interplay in depression: Metabolic insights and diagnostic potential.

Cell reports. Medicine·2026
Same author

Targeted degradation of CD24 by a transferrin receptor-engaging bispecific degrader enhances antitumor immunity.

Biochemical pharmacology·2026
Same author

Comparison between ex-situ and in-situ catalytic pyrolysis of food waste over nickel based catalysts.

BMC chemistry·2026
Same author

N6-Methyladenosine Modification of circIST1 Promotes Hypoxia-Inducible Factor α-mediated Glycolysis and Progression in Hepatocellular Carcinoma.

MedComm·2026

Related Experiment Video

Updated: Jan 30, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

9.3K

Emerging biosensor and assay-enabled high-throughput screening solutions for enzyme and strain engineering.

Jingyun Zhang1, Dan Zheng2, Sheena Chan3

  • 1Department of Biomedical Engineering, College of Design and Engineering, National University of Singapore, 119077, Singapore; NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), 117456, Singapore.

Current Opinion in Biotechnology
|January 28, 2026
PubMed
Summary
This summary is machine-generated.

Biosensors and assays are crucial for high-throughput screening (HTS) in enzyme and strain engineering. Integrating these tools with HTS accelerates biocatalyst discovery for sustainable biomanufacturing.

More Related Videos

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
06:51

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Published on: September 20, 2016

13.7K
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
07:37

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Published on: May 13, 2020

2.3K

Related Experiment Videos

Last Updated: Jan 30, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

9.3K
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
06:51

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Published on: September 20, 2016

13.7K
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
07:37

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Published on: May 13, 2020

2.3K

Area of Science:

  • Synthetic Biology
  • Biotechnology

Background:

  • High-throughput screening (HTS) platforms and biofoundries enable large-scale experimentation for enzyme and microbial strain engineering.
  • Biosensors and assays are essential for HTS, translating biochemical activities into measurable signals for performance evaluation.

Purpose of the Study:

  • To review recent progress and challenges in applying biosensor- and assay-enabled HTS for enzyme and strain libraries.
  • To discuss strategies for integrating diverse biosensor types with HTS.
  • To explore the role of biosensor-enabled HTS in machine learning-guided biocatalyst engineering.

Main Methods:

  • Review of recent literature on biosensor and assay development for HTS.
  • Discussion of integration strategies for various biosensor types (transcription factors, GPCRs, aptamers, etc.) with HTS.
  • Exploration of data generation for machine learning applications.

Main Results:

  • Limited availability and integration of robust biosensors/assays with HTS remain a bottleneck.
  • Diverse biosensor types can be integrated with HTS to detect a wide range of metabolites and products.
  • Biosensor-enabled HTS facilitates data generation for machine learning-guided biocatalyst engineering.

Conclusions:

  • Advances in biosensor- and assay-enabled HTS accelerate biocatalyst discovery.
  • These technologies are driving the next generation of sustainable biomanufacturing.
  • Overcoming integration challenges is key to unlocking the full potential of HTS in biocatalysis.