Related Experiment Video

Updated: Jan 30, 2026

Continuous Manual Exchange Transfusion for Patients with Sickle Cell Disease: An Efficient Method to Avoid Iron Overload
05:23

Continuous Manual Exchange Transfusion for Patients with Sickle Cell Disease: An Efficient Method to Avoid Iron Overload

Published on: March 14, 2017

20.4K

Identification of Sickle Cell Disease and Its Associated Other Structural Variants of Hemoglobin Using High

Dhirendra Kumar1, Lokeswara Bala Krishna S2, Sridevi Parikipandla3

  • 1Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, India.

Hemoglobin
|January 28, 2026
PubMed

Insights

Sickle cell disease (SCD) screening in India identified common Hb variants like HbSS, HbSF, and HbAS. Clinical profiling revealed increased severity with combined HbS and other hemoglobinopathies, particularly in females aged 21-30.

Area of Science:

  • Hematology
  • Medical Genetics
  • Population Health

Background:

  • Sickle cell disease (SCD) is a prevalent inherited blood disorder caused by hemoglobin mutations.
  • SCD disproportionately affects tribal populations, with a notable presence in Madhya Pradesh, India.
  • Understanding the spectrum of hemoglobinopathies associated with SCD is crucial for effective management.

Purpose of the Study:

  • To identify and characterize SCD-associated hemoglobinopathies, focusing on alpha (α) and beta (β)-globin structural variants.
  • To correlate hemoglobin variants with clinical severity and demographic factors in an Indian cohort.
  • To evaluate the utility of comprehensive hemoglobinopathy screening and clinical profiling in SCD management.

Main Methods:

  • Screening of over 1,200 symptomatic individuals for SCD.
  • Utilized solubility tests, complete blood count (CBC), alkaline electrophoresis, and high-performance liquid chromatography (HPLC) for sample analysis.
  • Detailed clinical profiling and comparative analysis of identified hemoglobin variants.

Main Results:

  • Identified high frequencies of HbSS (21.26%), HbSF (11.39%), and HbAS (63.73%) among positive samples.
  • Detected other significant variants including HbJ, Hb D-Punjab, and Hb Lepore.
  • HPLC analysis revealed numerous β-variants (99), α-variants (115), tetramers (12), and δβ-hybrid Hb variants (8). Increased severity was noted with combined HbS and other hemoglobinopathies, with a higher prevalence in females aged 21-30.

Conclusions:

  • Comprehensive hemoglobinopathy screening, including electrophoresis and HPLC, is effective in identifying diverse Hb variants in SCD patients.
  • Clinical profiling alongside genetic analysis provides critical insights into disease severity and patient stratification.
  • The findings underscore the importance of targeted screening programs in high-prevalence regions like tribal India for better SCD patient care.

Keywords:
Sickle cell diseaseclinical profilinghemoglobin variantshemoglobinopathieshigh performance liquid chromatography (HPLC)

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.7K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.4K

Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.5K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
3.0K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.5K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.7K
Hemoglobin01:24

Hemoglobin

Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well.
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
8.3K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
5.0K