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Related Experiment Video

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Cycling molecular assemblies for Golgi imaging and disruption.

Weiyi Tan1, Qiuxin Zhang1, Zhiyu Liu1

  • 1Department of Chemistry, Brandeis University, Waltham, MA, USA.

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Summary

Researchers developed cycling molecular assemblies (CyMA) for rapid Golgi imaging and cell-selective disruption. This novel approach utilizes self-assembling peptides for precise organelle targeting and functional interference.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Imaging

Background:

  • The Golgi apparatus is crucial for protein processing and transport.
  • Challenges exist in real-time Golgi imaging and targeted functional disruption.

Purpose of the Study:

  • To develop a method for rapid Golgi apparatus imaging.
  • To achieve cell-selective interference with Golgi functions.

Main Methods:

  • Utilized acetylated amphiphilic thiopeptides as precursors for cycling molecular assemblies (CyMA).
  • Exploited intracellular thioesterases and Golgi-resident palmitoyl acyltransferases for peptide modification and self-assembly.
  • Engineered CyMA with a biphenyl motif to disrupt Golgi functions.

Main Results:

  • Developed CyMA, dynamic nanostructures that enable near-instantaneous Golgi imaging via reversible S-acylation.
  • Demonstrated cell-selective disruption of Golgi functions including protein modification, trafficking, and secretion.
  • Achieved Golgi disruption leading to cell death by promoting CyMA accumulation.

Conclusions:

  • Dynamic supramolecular assembly offers a versatile strategy for Golgi-targeting.
  • CyMA enables pleiotropic interference with Golgi functions.
  • This approach may be adapted for targeting other organelles using alternative enzyme switches.