Mechanisms of metal ion-mediated quality regulation in rehydrated Salmon: Influence on texture, protein properties,
Xiuyi Jiang1, Chunhong Yuan2, Rui Yang3
1College of Food Science and Engineering, Ningbo University, Ningbo 315211, China; Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, Ningbo University, Ningbo 315211, China.
Abstract:
The present study compared the effects of equal concentrations of Na+, Ca2+, Mg2+, and Zn2+ on the rehydration performance, texture, and protein biochemical characteristics of freeze-dried salmon, both immediately after rehydration (0 days) and after rehydration following 3 weeks of storage at room temperature. The results showed that the metal ions had distinct effects on quality attributes. Na+ and Ca2+ helped maintain water-holding capacity and protein integrity, with Ca2+ enhancing protein network stability through electrostatic bridging. Mg2+ significantly improved the rehydration rate by promoting uniform water absorption, while Zn2+ increased hardness through protein cross-linking. SDS-PAGE and FTIR analyses indicated that Ca2+ and Mg2+ were most effective in preserving protein secondary structure and enzymatic activity, while also limiting lipid oxidation. This study clarified the differential regulatory roles of metal ions on protein conformation, microstructure, and oxidative stability, and provide a theoretical basis for quality control and formulation design of freeze-dried aquatic products.
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