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Updated: Jan 30, 2026
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Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
Published on: June 14, 2013
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Exosomal MicroRNA-1 Regulate Cx43 Expression via Tbx18 in Culture Atrial Fibroblasts under Rapid Electrical
Cheng-Yen Chuang1, Bao-Wei Wang1, Ying-Ju Yu1
1Division of Cardiology, Department of Internal Medicine.
Acta Cardiologica Sinica
|January 29, 2026
Summary
Rapid electrical stimulation affects microRNA-1 (miR-1) and T-box transcription factor 18 (Tbx18) in atrial fibroblasts, influencing connexin 43 (Cx43) expression. This reveals mechanisms for cardiac remodeling and potential targets for tachyarrhythmia.
Area of Science:
- Cardiovascular Biology
- Molecular Cardiology
- Biomedical Research
Background:
- MicroRNAs (miRs) play a role in cardiac remodeling.
- Tachyarrhythmia can alter microRNA expression.
- The impact of rapid electrical stimulation (RES) on fibroblast-derived exosomal miR-1 is not well understood.
Purpose of the Study:
- To investigate the molecular regulation of exosomal miR-1 in human atrial fibroblasts (HCF-aa) under RES.
- To explore the therapeutic potential of exosomal miR-1 in HCF-aa.
Main Methods:
- Human atrial fibroblasts (HCF-aa) were cultured and exposed to RES.
- miR-1 expression, T-box transcription factor 18 (Tbx18), and connexin 43 (Cx43) protein levels were analyzed.
- Luciferase reporter assays and immunohistochemical staining were employed.
Main Results:
- RES initially increased then decreased exosomal miR-1 expression.
- miR-1 overexpression reduced Tbx18 but increased Cx43; miR-1 antagomir decreased Cx43.
- miR-1 modulated Cx43 expression via Tbx18, confirmed by reporter assays and siRNA knockdown.
Conclusions:
- miR-1 and Tbx18 are key regulators of Cx43 expression in HCF-aa under RES.
- miR-1 influences Cx43 through Tbx18.
- These findings offer insights into cardiac remodeling mechanisms and potential therapeutic strategies for tachyarrhythmia.
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