Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autophagy01:27

Autophagy

5.8K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.8K
Binet's Contribution to Measures of Intelligence01:23

Binet's Contribution to Measures of Intelligence

1.7K
Alfred Binet, along with his student Théophile Simon, was tasked by the French Ministry of Education in 1904 to create a method for identifying students who struggled to learn through conventional classroom instruction. This initiative aimed to address overcrowding by placing such students in specialized schools. Binet and Simon developed an intelligence test comprising 30 tasks, ranging from simple commands, like touching one's nose or ear, to more complex tasks, such as drawing...
1.7K
What are Viruses?00:50

What are Viruses?

128.1K
Overview
128.1K
What is the Immune System?01:38

What is the Immune System?

127.8K
Overview
127.8K
Wechsler's Contribution to Measures of Intelligence01:23

Wechsler's Contribution to Measures of Intelligence

2.1K
David Wechsler, a psychologist who worked with World War I veterans, developed a significant IQ test in 1939 called the Wechsler-Bellevue Intelligence Scale. This test was innovative because it combined several subtests that measured both verbal and nonverbal skills, reflecting Wechsler's belief that intelligence is a global capacity involving purposeful action, rational thinking, and effective interaction with the environment. This test later evolved into the Wechsler Adult Intelligence...
2.1K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

692
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
692

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thymosin Alpha-1 Restores Chemotherapy-Induced Antitumor Immunity by Chaperoning a MicroRNA Ligand of TLR7 in Dendritic Cells.

Cancer research·2026
Same author

EcPSMB9 contributes to antiviral responses through autophagy-related and MHC I-associated processes and associates with EcPSMB8 in grouper.

Fish & shellfish immunology·2026
Same author

Machine learning-driven discovery of STAT1 and TRIM22 as immune biomarkers for lupus nephritis: translational insights into diagnosis and pathogenesis.

Scientific reports·2026
Same author

Prediction of Clinical Outcomes and Immunotherapy Response in Breast Cancer Based on T Cell-Mediated Tumor Killing-Related Traits.

Endocrine, metabolic & immune disorders drug targets·2026
Same author

Three-Dimensional Human Neurovascular Unit Modeling Reveals Cell-Specific Mechanisms of Traumatic Brain Injury.

Journal of functional biomaterials·2025
Same author

SLRanger: an integrated approach for spliced leader detection and operon prediction using long RNA reads.

Briefings in bioinformatics·2025

Related Experiment Video

Updated: Jan 31, 2026

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

7.7K

EcPSMB8 Contributes to Antiviral Immunity in Grouper by Modulating MHC I Signalling and Virus-Induced Autophagy.

Jinpeng Chen1, Xin He1, Bangsong Meng1,2

  • 1College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.

Journal of Fish Diseases
|January 29, 2026
PubMed
Summary

The study identifies EcPSMB8 in groupers as a key player in antiviral immunity. Overexpression of EcPSMB8 reduces virus replication by modulating autophagy and enhancing major histocompatibility complex class I signaling.

Keywords:
EcPSMB8MHC Iautophagyfish virusgrouper

More Related Videos

Assays for the Identification of Novel Antivirals against Bluetongue Virus
12:02

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Published on: October 11, 2013

14.6K
Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses
09:07

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Published on: January 20, 2017

10.6K

Related Experiment Videos

Last Updated: Jan 31, 2026

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

7.7K
Assays for the Identification of Novel Antivirals against Bluetongue Virus
12:02

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Published on: October 11, 2013

14.6K
Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses
09:07

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Published on: January 20, 2017

10.6K

Area of Science:

  • Immunology
  • Molecular Biology
  • Fish Aquaculture

Background:

  • The immunoproteasome subunit PSMB8 is vital in mammalian immunity.
  • Its function in fish antiviral defense, particularly in groupers, is largely unknown.

Purpose of the Study:

  • To clone and characterize the grouper PSMB8 homologue (EcPSMB8).
  • To investigate EcPSMB8's role in grouper antiviral immune responses.

Main Methods:

  • Cloning and expression analysis of EcPSMB8 in orange-spotted grouper.
  • Functional assays involving virus challenge (SGIV, RGNNV) and EcPSMB8 overexpression.
  • Analysis of autophagy markers (LC3-II) and autophagy-related genes.
  • Assessment of major histocompatibility complex class I (MHC I) signaling pathways (MHC I-A, ISRE, IFN3) and cytokine expression.

Main Results:

  • EcPSMB8 is ubiquitously expressed and localized in the cytoplasm.
  • EcPSMB8 overexpression significantly reduced SGIV and RGNNV replication.
  • Antiviral effects were linked to suppressed autophagy and enhanced MHC I signaling.
  • A specific polyclonal antibody against EcPSMB8 was successfully generated.

Conclusions:

  • EcPSMB8 plays a crucial role in grouper antiviral defense.
  • It acts by coordinating immune signaling and autophagy modulation.
  • EcPSMB8 is a potential target for enhancing fish antiviral immunity.