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An All-in-Tip Accelerated Proteomics Platform for Large-Scale Cancer Screening.

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A new automated All-in-Tip platform revolutionizes plasma proteomics, enabling deep profiling of over 3000 proteins per batch. This high-throughput, low-cost method accelerates biomarker discovery for precision medicine and cancer screening.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Analytical Chemistry

Background:

  • Plasma proteomics is crucial for human proteome atlas mapping and precision medicine.
  • Current methods face significant inefficiencies in sample processing, limiting large-scale application.

Purpose of the Study:

  • To develop a fully automated, room-temperature platform for high-throughput, cost-effective plasma proteomics.
  • To enhance the speed and depth of proteome profiling for clinical applications.

Main Methods:

  • Development of an All-in-Tip platform using functional macroporous nanoparticles (NPs).
  • Utilized NPs for rapid protein corona formation (5 min) to narrow dynamic range.
  • Employed spatial confinement within NPs to accelerate protein digestion (5 min).

Main Results:

  • Achieved deep proteome profiling (>3000 protein groups/batch) with high throughput (>4600 samples/day).
  • Demonstrated high reproducibility (Pearson's r > 0.91, CV < 20%).
  • Identified >5100 proteins in lung adenocarcinoma samples, distinguishing malignant from benign nodules (AUC 0.87-0.89).

Conclusions:

  • The All-in-Tip platform offers a rapid, in-depth, and high-throughput solution for plasma proteomics.
  • This technology can expedite cancer screening through biomarker discovery and mechanistic studies.
  • Holds promise for large-scale clinical cohort analysis in precision medicine.