Related Experiment Video
Updated: Jan 31, 2026
Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model
Published on: November 4, 2022
Effects of silybin on triptorelin-induced bone metabolic abnormalities in prostate cancer revealed based on TMT-based
Jianhui Li1, Xuejiao Lv1, Ying Jiang1,2
1Heilongjiang University of Chinese Medicine, Harbin, China.
Aims:
The aim of this study was to investigate the mechanism of action of SB on TRP-induced abnormalities of bone metabolism in LNCaP cells.
Methods:
The effects of different concentrations of SB and TRP alone and in combination on the proliferation of LNCaP cells were examined by CCK-8 assay, and the half maximal inhibitory concentration were screened for the subsequent experiments. Transwell migration and invasion assays were used to further investigate the effects of SB and TRP alone and in combination on the migration and invasion ability of LNCaP cells. Based on Tandem Mass Tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology, the differentially expressed proteins (DEPs) of LNCaP cells in the control group, SB group, TRP group and combination group were quantified. Bioinformatics technology was used to analyze the DEPs of LNCaP cells in each group. At last, the achieved key targets were verified by western blot.
Results:
The inhibitory effects of different concentrations of SB and TRP alone and in combination on the proliferation, migration and invasion of LNCaP cells showed both time-dependent and concentration-dependent effects, and the inhibitory effects of the combination of drugs on LNCaP cells were more significant. The proteomics results showed that a total of 153 DEPs were identified in the SB group and the control group, 100 DEPs were identified in the TRP group and the control group, and 524 DEPs were identified in the combination group and the control group. Bioinformatics analysis showed that a higher number of DEPs were enriched in the IL-17 signaling pathway in the SB and combined treatment groups compared to the control group. These findings suggest a potential role of SB and the combined treatment in modulating the IL-17 signaling pathway. In contrast, the same DEPs were found to be enriched in both the Circadian entrainment and Apelin signaling pathways in the TRP versus control and combined treatment versus control comparisons.
Conclusions:
SB may regulate TRP-induced bone metabolism abnormalities in LNCaP cells through the IL-17 signaling pathway as well as five DEPs: p-ERK2, RELA, HSP90B1, GNAI1and GNAI3.
Insights
SB and TRP inhibit LNCaP cell proliferation, migration, and invasion, with combined treatment being more effective. SB may regulate TRP-induced bone metabolism abnormalities via the IL-17 signaling pathway and specific proteins.
Area of Science:
- Molecular biology
- Cell biology
- Proteomics
Background:
- Prostate cancer bone metastases are a significant clinical challenge.
- Understanding the molecular mechanisms underlying bone metabolism abnormalities in prostate cancer is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the mechanism of action of SB on TRP-induced bone metabolism abnormalities in LNCaP cells.
- To identify key molecular targets involved in these processes.
Main Methods:
- Cell proliferation, migration, and invasion assays (CCK-8, Transwell).
- Proteomics analysis using Tandem Mass Tag (TMT) labeling and LC-MS/MS.
- Bioinformatics analysis of differentially expressed proteins (DEPs).
- Western blot validation of key targets.
Main Results:
- SB and TRP demonstrated dose- and time-dependent inhibition of LNCaP cell proliferation, migration, and invasion, with synergistic effects observed in combination.
- Proteomics identified significant numbers of DEPs, with 524 DEPs in the combination group.
- Bioinformatics analysis revealed enrichment of the IL-17 signaling pathway in SB and combination groups, while TRP and combination groups showed enrichment in Circadian entrainment and Apelin signaling pathways.
Conclusions:
- SB may modulate TRP-induced bone metabolism abnormalities in LNCaP cells through the IL-17 signaling pathway.
- Key differentially expressed proteins (DEPs) including p-ERK2, RELA, HSP90B1, GNAI1, and GNAI3 were identified as potential targets.
Related Concept Videos
02:23Abnormal Proliferation
00:52What is Metabolism?
02:33Lewis Acids and Bases
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
03:21Weak Base Solutions
02:54Ions as Acids and Bases
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
01:54Base Excision Repair
The first step of...