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Updated: Jan 31, 2026
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
Published on: October 8, 2019
Chirality-controlled biomolecular recognition in Cu(I) metallodrugs: the first enantiomorphic CuP2O2 systems with
S K Hadjikakou1, T M Koufou1, C N Banti1
1Biological Inorganic Chemistry Laboratory, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece. shadjika@uoi.gr.
Abstract:
Copper(II) nitrate reacts with tris(m-tolyl)phosphine (m-MePh)3P in 1 : 2 and 1 : 3 molar ratios to form [Cu((m-MePh)3P)2(NO3)] (1) which contains two isomers (1A and 1B) in the crystal lattice and [Cu((m-MePh)3P)3(NO3)] (2). The reaction of copper(II) nitrate with tris(p-tolyl)phosphine (p-MePh)3P in 1 : 3 molar ratio results in the {[Cu((p-MePh)3P)3(NO3)]·(DMF)·(H2O)} (3) complex. The complexes were characterized in the solid state using melting point (m.p), X-ray fluorescence spectroscopy (XRF), and attenuated total reflectance-Fourier transform infra-red (ATR-FT-IR) spectroscopy, and in solution using cryoscopy, ultraviolet-visible (UV-vis) and nuclear magnetic resonance (1H-NMR) spectroscopy. Their crystal structures were determined using single crystal X-ray crystallography in the solid state while the molecular weight was calculated using cryoscopy. A CCDC search shows that 1A and 1B are the first known chiral Cu(I) complexes of the CuP2O2 core. Binding affinity of complexes 1-3 toward calf thymus DNA (CT-DNA) was investigated ex vivo using UV-vis and fluorescence spectroscopy, viscosity measurements, and DNA denaturation assays. Their lipoxygenase (LOX) inhibitory activity was also studied. In silico computations further rationalized the DNA and LOX interactions with 1-3. In vitro assays were conducted to evaluate the activity of 1-3 against human breast adenocarcinoma (MCF-7) cells, and the results are presented herein.
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