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In situ Photo-rheology Monitors Viscoelastic Changes in Photo-responsive Polymer Networks
Published on: June 20, 2025
Naphthalene-Derived Photo-Switchable Hyperbranched Polymer by Anionic Polymerization for the Early Stage Detection
Narayan Das1, Tapendu Samanta1, Srishti Gautam1
1Polymer Research Centre, Department of Chemical Sciences, Indian Institute of Science Education and Research, Kolkata, India, West Bengal, PIN-741246, India.
Abstract:
The design and synthesis of macromolecules through controlled branching via hyperbranch polymerization have recently attracted attention from the scientific community due to their diverse biomedical applications, including diagnosis and drug delivery. This article highlights the development of a naphthalene-derived, photoswitchable, self-assembling hyperbranch polymer (PNap-Azo) in an aqueous medium, in which the azo bond serves as a stimulus-responsive feature in the presence of an overexpressed enzyme in a hypoxic microenvironment. PNap-Azo has a significant biological impact in hypoxic environments due to an azo bond (N═N), which transforms from a spherical to a twisted-rod shape upon cis-trans isomerization. Due to its amphiphilic nature and extensive hydrogen bonding, it exhibits an excellent twisted-rod morphology, enhancing the polymer's therapeutic efficacy in a hypoxic tumor environment. Therefore, PNap-Azo can detect and monitor azoreductase activity in a hypoxic environment through turn-on fluorescence responses, which are verified both spectroscopically and biologically in live cell lines. Our synthesized polymer, PNap-Azo, can monitor azoreductase activity in real time, potentially preventing complications before the condition worsens.
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