Related Experiment Video
Updated: Jan 31, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
Published on: July 13, 2012
Bioaugmentation of lyophilised composite hydrolytic and methanogenic inocula in an upscale continuous anaerobic
Ioannis A Fotidis1, Yixin Yan2, Dimitra S Pitsikoglou1
1Department of Hydraulics, Soil Science and Agricultural Engineering, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece; Department of Environment, Ionian University, 29100, Zakynthos, Greece.
Abstract:
Lignocellulose- and ammonia-rich feedstocks are abundant but remain underutilised in anaerobic digestion (AD) due to simultaneous inhibition of hydrolysis and methanogenesis. This study used, for the first time, a composite inoculum of hydrolytic bacteria and ammonia-tolerant methanogenic archaea, acclimatised, lyophilised, and stored long-term. When bioaugmented in an upscale continuous stirred-tank reactor, inhibition from high lignocellulose and ammonia levels was counteracted. Methane production increased by 145.05 ± 8.21 % relative to the inhibited phase, accompanied by stable biochemical conditions and restored methanogenic activity. Overall reactor production rate, before the addition of the biomasses and after bioaugmentation, increased by 46.79 ± 2.71 %. These short communication findings provide proof-of-concept that bioaugmentation with stored long-term composite inocula offers a scalable strategy to overcome lignocellulose and ammonia inhibition and enhance biogas production.
More Related Videos
11:49A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles
Published on: April 10, 2019
15:19Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Related Concept Videos
Phase I Reactions: Hydrolytic Reactions
An important hydrolytic reaction is ester hydrolysis. Ester bonds, often found in prodrugs, are broken down, increasing the solubility of drugs like aspirin and lidocaine for more straightforward elimination. Amide hydrolysis is another critical reaction, targeting amide bonds prevalent...
Classifying Matter by Composition
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures.
A pure substance is a form of matter that has a constant composition throughout with uniform properties. For example, any sample of sucrose has the same composition and same physical properties, such as melting point, color, and sweetness, regardless of the source from which it is isolated.
A mixture is composed of two or...
Continuing Care
Continuity of a Function
Continuity Equation
The mass flow rate is expressed as:
Continuity Equation