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Updated: Jan 31, 2026
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Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
Published on: April 17, 2012
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A green and efficient boronate affinity solid-phase extraction strategy for ginsenosides enrichment based on
Weiman Zhao1, Longchan Liu1, Wenxiang Fan1
1State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Journal of Chromatography. A
|January 29, 2026
Summary
A new boronic acid nanomaterial efficiently separates ginsenosides from plants. This green method enhances purification, yielding higher recovery and identifying more compounds than traditional techniques.
Area of Science:
- Natural Product Chemistry
- Materials Science
- Analytical Chemistry
Background:
- Ginsenosides, derived from Panax plants, possess significant bioactivities but are challenging to isolate.
- Traditional separation methods for ginsenosides are inefficient, costly, and environmentally unfriendly, using excessive organic solvents and complex processes.
Purpose of the Study:
- To develop an efficient and green solid-phase extraction (SPE) method for ginsenoside separation and purification.
- To utilize a novel boronic acid-functionalized dendritic mesoporous silica nanomaterial for ginsenoside isolation.
Main Methods:
- Construction of a boronic acid-functionalized mesoporous silica nanomaterial.
- Application of the nanomaterial as an SPE adsorbent for ginsenosides from Panax notoginseng decoction.
- Optimization of extraction parameters using single-factor experiments and Box-Behnken response surface methodology.
- Analysis of ginsenosides using High-Performance Liquid Chromatography with Charged Aerosol Detection (HPLC-CAD) and Ultra-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF-MS).
Main Results:
- The developed boron-affinity SPE strategy achieved enrichment factors 2.0 times higher than macroporous resin (3.4-4.0 vs. 1.7-2.0).
- High adsorption (93.5-98.7%) and desorption (88.9-97.9%) recoveries were obtained for major ginsenosides.
- UPLC-Q-TOF-MS analysis identified 127 ginsenosides, with significantly reduced non-ginsenoside interference.
- Greenness evaluations confirmed the environmental friendliness and sustainability of the boron-affinity strategy.
Conclusions:
- Boron-affinity SPE using functionalized nanomaterials offers a superior alternative for efficient and green ginsenoside purification.
- This approach is promising for the large-scale preparation of ginsenosides and other natural products containing cis-diol motifs.
- The method significantly improves separation efficiency, recovery rates, and compound identification compared to traditional techniques.
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