Related Experiment Video
Updated: Jan 31, 2026

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy
Published on: October 4, 2010
Enhancing atomic force microscopy stability through second harmonic optical fibre cavity control
Joana Nobre1, Tiago Cordeiro1, Tiago T Robalo1
1Departamento de Física, Faculdade de Ciências, Universidade de Lisboa, Campo Grande 16, 1749-016 Lisbon, Portugal; BioISI-Biosystems and Integrative Sciences Institute, Faculdade de Ciências, Universidade de Lisboa, Campo Grande 16, 1749-016 Lisbon, Portugal.
Researchers developed a new method to directly measure interatomic potentials by controlling tip-sample distance. This technique overcomes limitations of conventional atomic force microscopy, enabling precise force measurements.
Area of Science:
- Materials Science
- Surface Physics
- Nanotechnology
Background:
- Interatomic potentials govern material properties.
- Conventional atomic force microscopy (AFM) struggles to directly measure tip-sample interactions due to limited distance control.
- Existing methods often measure deflection versus displacement, not direct force-distance relationships.
Purpose of the Study:
- To propose and demonstrate a novel methodology for directly measuring tip-surface interactions as a function of tip-sample distance.
- To overcome the limitations of conventional AFM in quantifying interatomic potentials.
- To provide a more accurate approach for characterizing forces at the nanoscale.
Main Methods:
- Utilizing an AC interferometer to precisely monitor absolute tip displacement.
- Implementing a negative feedback loop to maintain constant tip position by adjusting the cantilever anchoring point.
- Actively controlling the tip-sample distance to a point of maximum interferometer sensitivity.
Main Results:
- Direct measurement of tip-surface interactions as a function of tip-sample distance.
- Successful prevention of tip-jump-to-contact phenomena, ensuring stable measurements.
- Accurate determination of indentation depth through sample motion tracking.
Conclusions:
- The proposed methodology offers a direct and accurate way to measure interatomic potentials.
- This technique enhances the capabilities of force microscopy by providing precise distance control.
- The findings pave the way for improved characterization of material properties at the atomic scale.
Related Concept Videos
Atomic Force Microscopy
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention
Nuclear Stability
To hold positively charged protons together...
Harmonic Mean
Take the example of the speed of a car, which is the measure of the rate of distance traveled. If the vehicle traverses the same distance back-and-forth, its average speed equals the total distance traveled divided by the total time taken. However, if the car moves with varying speeds, then the arithmetic mean is more skewed...
Intermolecular vs Intramolecular Forces
Atomic Orbitals

