Related Experiment Video
Updated: Jan 31, 2026

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy
Published on: October 4, 2010
Enhancing atomic force microscopy stability through second harmonic optical fibre cavity control
Joana Nobre1, Tiago Cordeiro1, Tiago T Robalo1
1Departamento de Física, Faculdade de Ciências, Universidade de Lisboa, Campo Grande 16, 1749-016 Lisbon, Portugal; BioISI-Biosystems and Integrative Sciences Institute, Faculdade de Ciências, Universidade de Lisboa, Campo Grande 16, 1749-016 Lisbon, Portugal.
Abstract:
The interatomic potential is key to understanding all properties of materials. Yet, conventional atomic force microscopes do not usually measure the interatomic potential, because they lack control of the tip-sample distance. Here, we propose a simple methodology for measuring tip-surface interactions directly as a function of tip-sample distance, rather than deflection as a function of sample displacement. We use an AC interferometer to monitor the absolute tip displacement. When a force is applied on the tip a negative feedback loop displaces the cantilever anchoring point, deflecting the lever such that the tip position remains constant. This feedback loop actively maintains the tip at a distance from an optical fibre where the interferometer sensitivity is maximum. As a result, the tip-sample distance or the indentation is directly given by the sample motion and the tip does not jump-to-contact.
Related Concept Videos
Atomic Force Microscopy
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention
Nuclear Stability
To hold positively charged protons together...
Harmonic Mean
Take the example of the speed of a car, which is the measure of the rate of distance traveled. If the vehicle traverses the same distance back-and-forth, its average speed equals the total distance traveled divided by the total time taken. However, if the car moves with varying speeds, then the arithmetic mean is more skewed...
Intermolecular vs Intramolecular Forces
Atomic Orbitals

