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Updated: Jan 31, 2026
Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments
Published on: March 21, 2016
Construction and characterization of 3D printable whey isolate protein-carrageenan emulsion gels incorporating
Yubo Zhao1, Xinyu Shi1, Qian Chen1
1College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Abstract:
Emulsion gels prepared with vegetable oils exhibit tunable structures and solid-like rheological properties. In this study, whey protein isolate (WPI)-κ-carrageenan (KG) emulsion gels were fabricated using five vegetable oils: corn oil, walnut oil, linseed oil, peanut oil, and olive oil. Gas chromatography revealed significant differences in unsaturated fatty acid content among the oils, which influenced protein adsorption at the oil-water interface and emulsion stability. The effects of oil type on gel properties were evaluated through microstructure analysis, rheological measurements, moisture distribution, and stability assessments. Results indicated that the linseed oil group (EG-Linseed), characterized by high linolenic acid (C18:3) content, exhibited smaller and more uniform droplet distributions, higher viscoelasticity, enhanced gel strength, and superior stability compared to other groups. Notably, the enhanced elastic dominance (G' > G″) and frequency-dependent viscoelasticity of the emulsion gels directly contributed to improved extrusion stability and shape retention during 3D printing. Low-field nuclear magnetic resonance (LF-NMR) analysis demonstrated elevated immobilized water content in EG-Linseed, indicative of a stable gel network. Furthermore, all emulsion gels displayed favorable 3D printability, storage stability, and thermal resistance, though freeze-thaw cycles induced structural breakdown. These findings provide insights into the role of fatty acid composition in modulating emulsion gel properties and support the development of plant-based fat substitutes for functional foods.
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