Related Experiment Video
Updated: Jan 31, 2026
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Magnetic aptasensor with UiO-MOFs-mediated orientation-ordered aptamers for sensitive portable test of okadaic acid
Qing'ai Chen1, Yuwei Zhou2, Guobin Huang2
1College of Tourism and Leisure Management, Fujian Business University, Fuzhou, Fujian, 350012, People's Republic of China.
Background:
Shellfish toxin residues (e.g., okadaic acid, OA) in seafood are a global concern, and the development of precise, sensitive portable test kits is therefore necessary. Magnetic nanoparticle-aptasensors (MNAS) are attractive for specific recognition and sensitive assay, yet direct epitaxial aptamer immobilization on Fe3O4 causes thin, scattered coatings, leading to limited probe capacity and non-specific adsorption. Fabricating high-efficiency MNAS with dense, ordered aptamer arrays for precise detection thus remains a challenging yet promising goal.
Results:
We propose a high-performance magnetic nanoparticle-aptasensor (MNAS) mediated by UiO-66-NH2 MOFs prepared with a secondary growth strategy (denoted as UiO/UiO-MOFs), in which AuNPs were uniformly anchored on MOF and facilitated the orientation-ordered nucleic acid aptamers enabling specific test of trace OA. The UiO/UiO-MOFs served as a scaffold with ordered amino group arrays, facilitating the construction of a homogeneous AuNPs interface and thus the tethering of high-density aptamers (up to 12.5 μmol/kg). This sensing platform synergistically integrated the advantages of ordered UiO/UiO-MOF self-assembly, robust AuNPs bridging, and the spherically ordered configuration of dense aptamers, thereby achieving efficient target OA recognition with minimal non-specific cross-reactivity. Coupled with laser-induced fluorescence (LIF) detection, the sensor exhibited excellent specificity, ultra-high sensitivity (limit of detection, LOD = 30 pg/mL), and outstanding stability. Notably, the sensor was successfully applied for the accurate on-site detection of OA residues in real mussel and clam samples, with satisfactory recovery rates ranging from 83.0 ± 3.3 % to 107.7 ± 1.3 %.
Significance:
This study posed an affordable yet powerful strategy capable of simultaneously realizing efficient UiO/UiO-MOFs surface mediation surface mediation and high capacity of spherical orientation-ordered aptamers. It expands the toolkit for epitaxial aptamer immobilization on MNAS, facilitates the ordered anchoring of aptamers, and thus enables the construction of advanced MNAS tailored for shellfish toxin detection.
Related Concept Videos
01:36Types of Toxins
Air pollutants, primarily gases, pose significant threats to respiratory health, leading to conditions like hypoxia, lung cancer, and in extreme cases, death.
Environmental pollutants like...
01:13Location and Orientation of the Heart
01:39Receptor-mediated Endocytosis
01:18Methods of Documentation I: Source-Oriented Records
In an SOR, each discipline involved in patient care maintains a separate medical record section. This record-keeping method enables easy tracking of patient progress and ensures healthcare staff have access to up-to-date information.
Key Attributes include the following:
03:02Colors and Magnetism
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
03:42Amino acids