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Published on: March 6, 2019
[Functional analysis of RcFUL in regulating flowering time in Rosa chinensis 'Old Blush']
1College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, Shaanxi, China.
Abstract:
Flowering is a critical phase in plant growth and development, regulated by a complex molecular network. The MADS-box transcription factor FRUITFULL (FUL) plays a significant role in controlling flowering time. In order to investigate its function and regulatory mechanism in Rosa chinensis, RcFUL was cloned from the flower buds of R. chinensis 'Old Blush'. Quantitative real-time PCR polymerase results revealed that RcFUL was predominantly expressed in the pistil, and its expression gradually increased during floral development in this variety. Subcellular localization in tobacco showed that RcFUL localized in the nucleus. Silencing RcFULvia virus-induced gene silencing resulted in delayed flowering in TRV-RcFUL plants. Yeast one-hybrid and dual-luciferase reporter assays confirmed that RcSPL1 directly bound to the promoter region of RcFUL. These findings reveal the crucial role of RcFUL in regulation of the flowering time of R. chinensis,expanding the regulatory network underlying the flowering and providing new theoretical insights for the molecular breeding of R. chinensis.
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