Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

8.5K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.5K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

14.5K
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
14.5K
Nuclear Fusion02:45

Nuclear Fusion

33.8K
The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons.
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
33.8K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.2K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.2K
Encoding01:19

Encoding

845
Information enters the brain through encoding, which is the input of information into the memory system. Once sensory information is received from the environment, the brain labels or codes it. The information is then organized with similar information and connected to existing concepts. Encoding occurs through automatic processing and effortful processing.
Automatic processing involves the encoding of details like time, space, frequency, and the meaning of words, usually done without conscious...
845
Elastin is Responsible for Tissue Elasticity01:12

Elastin is Responsible for Tissue Elasticity

3.2K
Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mussel Adhesive Protein/Hyaluronic Acid Hydrogels for EGF Delivery and MRSA-Infected Diabetic Wound Repair.

Gels (Basel, Switzerland)·2026
Same author

Cosolvent-induced spontaneous refolding of lipase.

Applied microbiology and biotechnology·2026
Same author

Tailoring Regioselectivity-Controlled UDP-Glycosyltransferase for Bidirectional Glycosylation of Tyrosol via Free Energy-Driven Pocket Reshaping and Tunnel Engineering.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Artificial intelligence-assisted next-generation biomaterials: From design and preparation to medical applications.

Colloids and surfaces. B, Biointerfaces·2025
Same author

Supramolecular Prodrug Hydrogel for One-Week Protection Against Thrombosis.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Scale up of fermentation of recombinant Escherichia coli for efficient production of spider drag silk protein MaSp1s and its dimers.

Microbial cell factories·2025

Related Experiment Video

Updated: Jan 31, 2026

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
07:35

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Published on: June 9, 2014

22.5K

Genetically Encoded Multivalent Elastin-Like Polypeptide-Haemadin Fusion Proteins for Prolonged Antithrombotic

Hui Yang1,2, Lianlian Liang1, Shuo Su1

  • 1School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, China.

Biomacromolecules
|January 30, 2026
PubMed
Summary

We engineered a fusion protein combining haemadin (a thrombin inhibitor) with elastin-like polypeptides (ELPs) for sustained antithrombotic therapy. This platform provides on-demand drug release, improving peptide therapeutic safety and convenience.

More Related Videos

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
08:20

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Published on: March 28, 2016

8.3K
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

13.1K

Related Experiment Videos

Last Updated: Jan 31, 2026

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
07:35

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Published on: June 9, 2014

22.5K
Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
08:20

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Published on: March 28, 2016

8.3K
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

13.1K

Area of Science:

  • Biotechnology
  • Pharmacology
  • Biochemistry

Background:

  • Peptide therapeutics face limitations due to short plasma half-lives, requiring frequent dosing.
  • This leads to pharmacokinetic variability and potential side effects.
  • Developing strategies for sustained and controlled peptide delivery is crucial for clinical success.

Purpose of the Study:

  • To create a genetically encodable fusion protein platform for sustained and stimuli-responsive peptide drug delivery.
  • To combine a thrombin inhibitor (haemadin) with an elastin-like polypeptide (ELP) for enhanced antithrombotic therapy.
  • To enable on-demand activation of therapeutic activity and improve dosing convenience.

Main Methods:

  • Developed a fusion protein linking haemadin and ELP via factor Xa (FXa)-cleavable linkers.
  • Utilized subcutaneous injection for in situ depot formation via temperature-triggered ELP condensation.
  • Achieved high-yield expression and nonchromatographic purification of the fusion protein.

Main Results:

  • The fusion protein formed an in situ depot upon subcutaneous injection, enabling slow release of an inactivated prodrug.
  • Thrombus-associated FXa cleaved the linker, liberating active haemadin specifically at the site of thrombosis.
  • Subcutaneous administration significantly prolonged antithrombotic protection postinjection.

Conclusions:

  • The developed fusion protein platform offers tunable drug loading, long-term release, and stimuli-responsive activation.
  • This approach enhances the safety, efficacy, and dosing convenience of peptide therapeutics.
  • The technology shows strong potential for improving the clinical application of peptide-based drugs.