Related Experiment Video
Updated: Jan 31, 2026
07:07
Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
11.9K
Strontium-doped hydroxyapatite microspheres loaded with iloprost promote dentin-pulp complex regeneration
Jilong Chen1, Jingyi Di1, Qiming Yu1
1Department of Stomatology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Frontiers in Bioengineering and Biotechnology
|January 30, 2026
Summary
This study developed Ilo@Sr-HA, a novel biomaterial for vital pulp therapy (VPT). Ilo@Sr-HA significantly enhanced pulp repair and dentin regeneration in animal models, showing promise for future dental applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Dental Research
Background:
- Vital pulp therapy (VPT) aims to maintain pulp vitality but current materials like calcium hydroxide and MTA have limitations.
- Developing advanced biomaterials is crucial for enhancing dentin-pulp complex regeneration.
- Strontium-doped hydroxyapatite (Sr-HA) and pro-angiogenic agents show potential for improved pulp repair.
Purpose of the Study:
- To synthesize and characterize strontium-doped hydroxyapatite (Sr-HA) microspheres.
- To develop a novel biomaterial, Ilo@Sr-HA, by loading iloprost onto Sr-HA for enhanced pulp regeneration.
- To evaluate the efficacy of Ilo@Sr-HA in promoting dentin-pulp complex repair both in vitro and in vivo.
Main Methods:
- Synthesized hollow hydroxyapatite microspheres with varying Sr concentrations (5%, 10%, 15%).
- Identified optimal 5% Sr-HA concentration based on ion release and cytocompatibility.
- Loaded iloprost onto 5% Sr-HA to create Ilo@Sr-HA and assessed its properties and effects on human dental pulp stem cells (hDPSCs).
- Evaluated Ilo@Sr-HA in a rat pulp-exposure model using micro-CT and histological analysis.
Main Results:
- 5% Sr-HA demonstrated optimal ion release and cytocompatibility; Ilo@Sr-HA showed biphasic release without cytotoxicity.
- Ilo@Sr-HA significantly enhanced hDPSCs proliferation, ALP activity, and odontogenic gene expression (DMP1, DSPP) in vitro.
- In vivo studies showed Ilo@Sr-HA promoted superior reparative dentin formation and continuous dentin bridges compared to Dycal.
- Ilo@Sr-HA group exhibited reduced inflammation and better pulp preservation in the rat model.
Conclusions:
- Ilo@Sr-HA effectively combines Sr2+ ionic cues and iloprost's effects to create a bioactive environment for pulp repair.
- The developed Ilo@Sr-HA biomaterial shows significant potential for advancing vital pulp therapy and dentin-pulp regeneration.
Related Concept Videos
00:50Anaphase Promoting Complex
3.4K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.4K
02:40The Eukaryotic Promoter Region
18.8K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
02:40The Eukaryotic Promoter Region
3.9K
3.9K
01:57Protein Complexes with Interchangeable Parts
2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
01:19Impact Loading
693
Impact loading occurs when a moving object collides with a stationary structure, such as a rod with a uniform cross-sectional area fixed at one end. Under these conditions, the rod absorbs the kinetic energy from the striking object, leading to deformation and subsequent stress development. As the rod returns to its original position and reaches maximum stress, the absorbed energy, initially manifested as kinetic energy, transforms entirely into strain energy.
In cases of elastic deformation,...
In cases of elastic deformation,...
693
02:41Protein Complex Assembly
16.7K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.7K