Related Experiment Video
Updated: Jan 31, 2026
10:16
Passaging Human Neural Stem Cells
Published on: August 22, 2007
15.3K
Cultured cells activate IRE1 during attachment and flattening after routine passaging
Paige Dillon1, Lincoln Hollingshead1, Julie Hollien1
1School of Biological Sciences, University of Utah, Salt Lake City, Utah, United States.
Micropublication Biology
|January 30, 2026
Summary
Routine cell passaging triggers Xbp1 splicing, activating the unfolded protein response. This occurs independently of ER stress, suggesting cells initiate this pathway during reattachment and spreading after subculturing.
Area of Science:
- Cellular biology
- Molecular biology
- Biochemistry
Background:
- Endoplasmic reticulum (ER) stress typically activates the unfolded protein response (UPR) via IRE1-mediated Xbp1 mRNA splicing.
- This process generates a transcription factor crucial for restoring proteostasis.
Purpose of the Study:
- To investigate whether Xbp1 splicing occurs during routine cell culture procedures without artificial ER stressors.
- To determine the factors influencing Xbp1 splicing during cell passaging.
Main Methods:
- Culturing mouse MC3T3-E1 cells.
- Performing routine cell passaging using various dissociation buffers.
- Assessing Xbp1 splicing.
- Utilizing non-adherent culture dishes.
Main Results:
- Xbp1 splicing was observed in cultured cells during routine passaging, independent of ER stress.
- The dissociation buffer type did not affect Xbp1 splicing.
- Splicing was reduced when cells were cultured on non-adherent surfaces.
Conclusions:
- Cultured mammalian cells can initiate the unfolded protein response during the mechanical and adhesive processes of reattachment and spreading post-passaging.
- Cellular microenvironment and physical cues during passaging can influence UPR activation.
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