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Updated: Jan 31, 2026

Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction
Published on: July 8, 2017
Monitoring presynaptic calcium dynamics with membrane-impermeant fluorescent indicators in motor nerve endings
Dmitry V Samigullin1,2, Ellya A Bukharaeva1
1Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, P.O. box 261, Kazan, 420111 Russia.
Abstract:
Calcium entry through voltage-gated calcium channels as well as calcium release from intracellular calcium depots is crucial for triggering the release of neurotransmitters, synaptic plasticity and regulating the activity of various intracellular proteins. Recent evidence suggests that neuronal calcium signaling is impaired in many neurodegenerative disorders. This review presents methodological approaches that allow the estimation of the dynamics of calcium entry in motor nerve endings using fluorescent calcium indicators. The methods considered were: selecting calcium dyes depending on the experimental conditions, and loading indicators into the cells of interest. This review outlines general principles and techniques to record fast fluorescent calcium signals. Possible results that can be obtained within the framework of experiments using the described methods are also presented.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s12551-025-01336-4.
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