Related Experiment Video
Updated: Jan 31, 2026

Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes
Published on: February 8, 2018
In Vivo Evaluation of Liver Injury and Arthritis Enabled by a Nitroxyl-Activated Near-Infrared Fluorescence and
Lejuan Liao1, Yidong Bin1, Zongyi He1
1State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Abstract:
Nitroxyl (HNO) is the transient form of nitric oxide (NO) and is highly reactive, which makes its in vivo study a significant challenge. We report here simultaneous fluorescence and photoacoustic imaging of HNO, enabled by a newly synthesized near-infrared, dual-modality fluorescence and photoacoustic (FPA) probe. The 2-(diphenylphosphonyl)-benzoate moiety in the FPA probe specifically recognized HNO. The photophysical properties of the probe were tuned, e.g., by substituting an oxygen atom in the hemicyanine moiety with sulfur, resulting in a 45 nm red-shift. The maximum absorption wavelength increased from 650 nm in the absence of HNO to 760 nm in the presence of HNO. We demonstrate an application of the HNO-activated FPA probe to in vivo evaluation of inflammatory disease progression in a mouse model. Treatment of mice with acetaminophen stimulated endogenous generation of HNO in their liver, which was successfully imaged using dual-modality near-infrared fluorescence and photoacoustic measurements. Fluorescence and photoacoustic imaging of the ankle site of an arthritis mouse model enabled a study of the onset of this inflammatory disease.
More Related Videos
06:46Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
Published on: May 8, 2017
09:43In vivo Near Infrared Fluorescence NIRF Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis
Published on: August 4, 2011
Related Concept Videos
Sensory Modalities
General senses refer to the broad category of sensory information detected by receptors in the body and can be further grouped into somatic and visceral senses. Somatic sensations include touch, pressure, temperature, and pain and are essential for navigating our environment and...
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...
Liver Histology
Hepatocytes perform a variety of essential functions. They secrete...
Liver Physiology
Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of 70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can...
Liver Regeneration
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...