Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.7K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.7K
Peptide Bonds02:43

Peptide Bonds

82.9K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
82.9K
Need for Obtaining Pure Cultures01:29

Need for Obtaining Pure Cultures

1.6K
Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
1.6K
Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

2.4K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
2.4K
Energy-releasing Steps of Glycolysis01:28

Energy-releasing Steps of Glycolysis

146.8K
Glycolysis is divided into two phases based on whether energy is utilized or released. While the first phase consumes ATP, the second phase produces energy in the form of ATP and NADH. The energy is released over a sequence of reactions that turns G3P into pyruvate. The energy-releasing phase—steps 6-10 of glycolysis—occurs twice, once for each of the two 3-carbon sugars produced during steps 1-5 of the first phase.
The first energy-releasing step—the 6th step of glycolysis...
146.8K
ATP Energy Storage and Release01:31

ATP Energy Storage and Release

14.4K
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
14.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated Radiosynthesis of High-Molar-Activity 4-Borono-2-[<sup>18</sup>F]Fluorophenylalanine: Validation in Rodent Tumor Models and Nonhuman Primates.

Molecular pharmaceutics·2026
Same author

Conformationally Tuned Cyclic RGD Peptides for Integrin-Subtype-Selective PET/CT Imaging.

Journal of medicinal chemistry·2026
Same author

Design, Synthesis, and Evaluation of [<b><sup>68</sup></b>Ga/<b><sup>177</sup></b>Lu]-Labeled TSPO-Targeting Radioligands for PET/CT Diagnosis and Radionuclide Therapy of Brain Tumor.

Journal of medicinal chemistry·2026
Same author

Development and Preclinical Evaluation of the Novel Peptide-Based Tracer <sup>68</sup>Ga-PDT01 for Noninvasive PET Imaging of PD-L1 Expression.

Journal of medicinal chemistry·2026
Same author

Postmodification of Daptomycin at Tryptophan Enables the Discovery of Aryl-Daptomycin Antibiofilm Activity against MRSA.

Journal of medicinal chemistry·2026
Same author

Tyrosine-Selective Polyfluoroaryl Modification Enables Bioactive Peptides with Enhanced Membrane Permeability and Ligation Capability.

Organic letters·2026

Related Experiment Video

Updated: Jan 31, 2026

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
10:58

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

10.7K

Highly Pure Combinatorial Peptide Libraries by Resin-Enabled Catch and Release.

Quan Zuo1, Jie Yan1, Hongyi Huang1

  • 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Xian Nong Tan Street, Beijing 100050, P. R. China.

JACS Au
|January 30, 2026
PubMed
Summary

A new N-terminal cysteine-based dynamic catch-and-release (CbDCR) platform purifies peptide libraries, enabling the discovery of high-affinity ligands for peptide-drug conjugate (PDC) development and diagnostic applications.

Keywords:
N-terminal cysteinecatch-and-releasecombinatorial peptide librariesfunctionalized resinpeptide−drug conjugates

More Related Videos

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology
11:42

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

25.3K
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

18.8K

Related Experiment Videos

Last Updated: Jan 31, 2026

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
10:58

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

10.7K
Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology
11:42

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

25.3K
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

18.8K

Area of Science:

  • Medicinal Chemistry
  • Bioconjugation Chemistry
  • Peptide Synthesis

Background:

  • High-quality peptide ligands are crucial for peptide-drug conjugate (PDC) development.
  • Combinatorial peptide libraries are vital for discovering high-affinity ligands but are often plagued by low purity and false positives.
  • Efficient purification methods are needed to overcome limitations in current peptide library synthesis and screening.

Purpose of the Study:

  • To develop a novel purification platform for N-terminal cysteine-containing peptides.
  • To enable efficient library preparation and purification for high-affinity ligand discovery.
  • To facilitate the development of peptide-drug conjugates and diagnostic agents.

Main Methods:

  • Development of an N-terminal cysteine-based dynamic catch-and-release (CbDCR) platform.
  • Utilized a recyclable 2-formylphenylboronic acid (2FPBA) resin for pH-responsive peptide capture and release.
  • Applied the CbDCR platform for solid-phase peptide synthesis (SPPS) purification, enrichment from protein lysates, and split-and-pool library preparation.
  • Integrated the platform with microplate-based high-throughput workflows.

Main Results:

  • The CbDCR platform demonstrated high purification efficiency for N-terminal cysteine-containing peptides.
  • Successfully purified SPPS-synthesized peptides and enriched peptides from protein lysates.
  • Enabled high-purity split-and-pool library preparation for ligand discovery.
  • Identified high-affinity integrin αvβ6-targeting peptides using a purified RGD-focused library.
  • Constructed three peptide-radionuclide conjugates for potential pancreatic cancer diagnostics.

Conclusions:

  • The CbDCR platform offers a simple, scalable, and efficient method for purifying peptide libraries.
  • This technology accelerates the discovery of high-value peptide ligands and the development of PDCs.
  • The CbDCR platform streamlines multiple stages of peptide-based drug and diagnostic development.