Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Salt Stress02:02

Responses to Salt Stress

14.6K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.6K
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

14.8K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.8K
Stress Response System01:21

Stress Response System

879
The stress response system, also known as the fight-or-flight response, is the body's automatic physiological reaction to perceived threats. Hans Selye introduced the concept of General Adaptation Syndrome (GAS) to describe the predictable pattern of changes that occur in response to stress. GAS consists of three sequential stages: alarm, resistance, and exhaustion. This model helps explain how chronic stress can contribute to health problems.
Alarm stage
In the alarm stage, the body's...
879
Psychological Responses to Stress01:20

Psychological Responses to Stress

692
Psychological responses to stress encompass the various cognitive and emotional reactions individuals experience when faced with challenging or threatening situations, such as a job loss. Prolonged exposure to stressors can disturb emotional balance, increasing negative emotions (e.g., anxiety and sadness) and diminishing positive emotions (e.g., joy and satisfaction). These persistent emotional shifts are associated with an increased risk of both physical illness and mental health issues, such...
692
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

395
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
395
Yeast Signaling01:28

Yeast Signaling

17.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The priority of yeast to select among various DNA options to repair genome breaks by homologous recombination.

Molecular biology reports·2024
See all related articles

Related Experiment Video

Updated: Jan 31, 2026

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
12:31

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

14.8K

Yeast Stress Response to Synthetic Constructs.

Musa Tartik1,2

  • 1Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bingol University, Bingol 12000, Turkey.

ACS Synthetic Biology
|January 30, 2026
PubMed
Summary

Engineering yeast with synthetic biology constructs disrupts cellular balance, activating stress responses. This review details these stresses and proposes strategies for creating more resilient and productive Saccharomyces cerevisiae strains for biotechnology.

Keywords:
Saccharomyces cerevisiaemetabolic burdenstressomesynthetic biologyyeast stress response

More Related Videos

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
11:04

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Published on: June 23, 2018

7.7K
Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

15.0K

Related Experiment Videos

Last Updated: Jan 31, 2026

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
12:31

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

14.8K
Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
11:04

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Published on: June 23, 2018

7.7K
Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

15.0K

Area of Science:

  • Synthetic biology
  • Biotechnology
  • Cellular stress response

Background:

  • Saccharomyces cerevisiae is a key chassis in synthetic biology.
  • Heterologous constructs commonly disrupt cellular homeostasis, including proteostasis, metabolism, redox balance, and secretion.
  • These disruptions trigger complex stress pathways, collectively termed the 'stressome', which maintains cellular integrity but limits productivity.

Purpose of the Study:

  • To review construct-induced stress in engineered yeast.
  • To present stress-aware design principles for improving yeast strain robustness and yield.
  • To guide the development of advanced Saccharomyces cerevisiae systems for biotechnology.

Main Methods:

  • Literature review of stress pathways activated by heterologous constructs in yeast.
  • Summary of current strategies for mitigating cellular burden and stress.
  • Overview of emerging methods for stress mitigation and strain engineering.

Main Results:

  • Engineered yeast activates multiple stress pathways (heat shock, unfolded protein response, oxidative stress, etc.).
  • The 'stressome' balances homeostasis and productivity, posing a challenge for synthetic biology.
  • Various strategies can reduce cellular burden and improve stress tolerance.

Conclusions:

  • Understanding the interaction between synthetic designs and yeast stress pathways is crucial.
  • Implementing stress-aware design principles can lead to more resilient and higher-yielding yeast.
  • Advanced engineering methods offer new avenues for optimizing Saccharomyces cerevisiae for biotechnological applications.