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Updated: Feb 1, 2026
Objective Nociceptive Assessment in Ventilated ICU Patients: A Feasibility Study Using Pupillometry and the Nociceptive Flexion Reflex
Published on: July 4, 2018
Prognostic Value and Potential Mechanism of CD8+ T Subsets Phenotype in ICU Patients with Sepsis
Guoyu Zhao1, Hao Wang2, Jiahui Zhang1
1Department of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Background:
While CD8+ T-cell subsets play a crucial role in sepsis prognosis, the prognostic value of their dynamic changes and molecular mechanisms remains incompletely understood. Compared to static single-timepoint measurements, the prognostic role of repeated measurements over time remains undetermined.
Methods:
The study included 220 intensive care unit patients with sepsis (2023.08-2024.05). K-means clustering analysis based on CD8+CD28+/CD8+%, CD8+CD38+/CD8+%, and CD8+DR+/CD8+% was used to categorize patients into two groups. Clinical data were collected and analyzed for 28-day mortality prediction. Fifteen patients from each group were selected for Olink proteomics analysis to investigate differentially expressed proteins and conduct functional enrichment analysis.
Results:
Using K-means clustering analysis based on CD8+ T-cell subset phenotypes, patients were divided into group 1 (n = 74) and group 2 (n = 146), with group 2 demonstrating significantly higher 28-day mortality compared to group 1 (32.3% vs. 17.6%, P < 0.001). Further dynamic trend analysis revealed that patients exhibiting continuously decreasing CD8+CD28+/CD8+% and increasing CD8+CD38+/CD8+% had the poorest prognosis, with a 28-day mortality rate of 50%. The combined measurement of CD8+CD28+/CD8+% and CD8+CD38+/CD8+% showed enhanced prognostic value (area under the curve = 0.676) compared to individual measurements, indicating the importance of monitoring multiple T-cell subset markers. Additionally, Olink proteomic analysis identified IL-12Rβ1 as a key molecular mediator closely associated with CD8+ T-cell function and activation subsets, providing insights into the underlying mechanism of immune dysfunction in sepsis patients.
Conclusions:
CD8+ T-cell subset phenotypes serve as important prognostic indicators in sepsis, with IL-12Rβ1 potentially being a key molecule affecting CD8+ T-cell function, providing a new potential target for sepsis monitoring and immunotherapy.
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