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Unveiling the Interactions between a Protein Derived from Barnacle Adhesive and Surfaces Using Surface Plasmon
Dhekra Ayed1,2, Zahraa Khalil1,3, Cédric R Picot3
1University Grenoble Alpes, CNRS, Grenoble-INP, LMGP, 38000 Grenoble, France.
ACS Applied Bio Materials
|January 30, 2026
Summary
Barnacle-inspired M19-2 protein shows strong adsorption on various surfaces, especially at acidic pH. This research offers insights into bioinspired adhesives and protein-interface interactions.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Biophysics
Background:
- Protein adsorption at interfaces is crucial in biological and engineered systems.
- Understanding protein-interface interactions under varying conditions (pH, ionic strength, temperature) remains a challenge.
- Barnacle adhesive proteins offer insights into bioadhesion in wet environments.
Purpose of the Study:
- To investigate the adsorption behavior of a barnacle-inspired recombinant protein, M19-2.
- To explore the influence of surface properties, pH, temperature, and ionic strength on M19-2 adsorption.
- To compare M19-2 adsorption with model proteins like fibrinogen, BSA, and lysozyme.
Main Methods:
- Production and purification of recombinant M19-2 protein in *Escherichia coli*.
- Utilizing surface plasmon resonance imaging (SPRi) to study protein adsorption.
- Systematically varying pH, temperature, and ionic strength to assess binding characteristics.
Main Results:
- M19-2 exhibited enhanced adsorption at acidic pH across diverse self-assembled monolayer (SAM) surfaces.
- Adsorption was influenced by temperature, ionic strength, pH, and protein concentration.
- M19-2 demonstrated stronger adsorption relative to its size compared to model proteins.
Conclusions:
- M19-2 shows significant potential as a bioinspired adhesive molecule.
- The study elucidates key factors governing protein adsorption on different surfaces.
- Findings contribute to the development of advanced bioinspired adhesive materials.
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