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Updated: Feb 1, 2026

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Resveratrol inhibits bladder cancer proliferation by targeting the AURKA/STAT3 axis: From computational analysis to

Chao Feng1, Guodong Chen1, Yan Shu1

  • 1Department of Urology, Tongde Hospital of Zhejiang province, Hangzhou, Zhejiang, China.

Plos One
|January 30, 2026
PubMed
Abstract

Insights

Resveratrol inhibits bladder cancer (BCa) by targeting the AURKA/STAT3 pathway. This natural compound suppresses BCa cell proliferation and induces apoptosis, offering a promising therapeutic strategy.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Bladder cancer (BCa) has a high recurrence rate and conventional treatments cause adverse effects.
  • Resveratrol (Res) shows cytotoxicity against various tumors, but its mechanism in BCa requires further study.
  • Identifying novel therapeutic targets and natural compounds is crucial for BCa treatment.

Purpose of the Study:

  • To investigate the mechanism of Res in inhibiting BCa cells.
  • To explore the role of the AURKA/STAT3 axis in Res-mediated BCa suppression.
  • To provide a theoretical basis for Res as a potential BCa therapeutic agent.

Main Methods:

  • Bioinformatics analysis to identify differentially expressed genes and Res binding sites.
  • CCK8 assays to determine Res concentrations for 30% and 50% inhibition.
  • Flow cytometry for cell cycle and apoptosis analysis.
  • Immunofluorescence, Western blot, and qPCR to assess protein and gene expression.

Main Results:

  • Resveratrol demonstrated time- and dose-dependent inhibition of BCa cell survival and cell cycle progression.
  • Computational analysis identified AURKA as a potential Res target.
  • Both Resveratrol and MLN8237 suppressed STAT3 expression in BCa cells.
  • Resveratrol and MLN8237 inhibited the expression of AURKA and downstream genes (VEGF, Bcl-2, Cyclin D1).

Conclusions:

  • Resveratrol regulates BCa cell expression via the AURKA/STAT3 axis.
  • These findings support Resveratrol as a potential therapeutic agent for bladder cancer.
  • This study provides a foundation for developing multi-target drugs based on Resveratrol structure.

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