NuSAP Safeguards Centriole Integrity to Mediate CEP57-CEP152 Torus Recruitment for Proper Engagement

Precise centrosome regulation is crucial for faithful cell division, yet how microtubule-associated proteins (MAPs) contribute to centrosome integrity remains poorly understood. Here, we uncover a novel localization of NuSAP-a RanGTP-regulated MAP-on centrioles using super-resolution microscopy. NuSAP depletion disrupts centriole tubulin architecture, triggering premature centriole disengagement, compromising pericentriolar material (PCM) cohesion, and disrupting the spatial organization of the CEP57-CEP63-CEP152 torus assembly. Using TurboID-based proteomics and biochemical assays, we identify CEP57 as a direct interactor of NuSAP. We further demonstrate that NuSAP is essential for the initial recruitment of the CEP57-CEP63-CEP152 complex to the proximal end of procentrioles. Based on these findings, we propose a novel two-step model of torus complex recruitment, in which NuSAP-dependent stabilization of centriole tubulin is required for early CEP57 loading. This discovery not only resolves a longstanding gap in our understanding of how MAP-mediated tubulin stabilization contributes to centrosome organization but also provides a mechanistic framework for the hierarchical recruitment of the centriole torus complex.