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Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

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Published on: May 12, 2017

Protective effect of Lonicera similis Hemsl leaves granules on the intestinal tract of heat-stressed broilers

Dai-Zheng Yin¹, Lin-Ge Zhang¹, Jing Yang²

¹Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, PR China.

Journal of Ethnopharmacology

|January 30, 2026

Abstract

Ethnopharmacological Relevance:

Lonicera similis Hemsl leaves (LSHL), a widely distributed traditional Chinese herbal remedy, possesses anti-inflammatory properties. However, its regulatory role in mitigating heat stress-induced intestinal impairment in broilers remains inadequately elucidated.

Aim Of The Study:

This study identified LSHL's active components, established a broiler heat stress model, and investigated its intestinal protective effects and mechanisms.

Materials And Methods:

Liquid chromatography-mass spectrometry (LC-MS) analyzed LSHL's active components. Network pharmacology predicted mechanisms, and molecular docking verified key component-target interactions. In vivo experiments evaluated LSHL's effects via growth performance, immune organ indices, histopathology, antioxidant enzyme activities, inflammatory cytokine levels, and qPCR.

Results:

LC-MS identified 42 active components (e.g., Alizarin, Caffeic Acid, Quinic Acid). Network pharmacology revealed 41 overlapping targets (core: ESR1, CASP3, MAPK10) and involvement of the MAPK signaling pathway. Molecular docking confirmed strong component-target binding. Dietary LSHL granules (1-3 g/kg) improved growth performance, alleviated intestinal villi damage, reduced oxidative stress (decreased MDA; increased GSH-Px, CAT), attenuated inflammation (decreased TNF-α, IL-6, IL-1β), upregulated immunoglobulins (IgG, IgM, IgA), and downregulated HSP70 and MAPK pathway-related proteins.

Conclusions:

LSHL exerts multi-component and multi-target protective effects on the intestinal tract of heat-stressed broilers. These findings provide a theoretical foundation for the application of LSHL as a natural anti-heat stress feed additive.

Keywords:

Heat stress Lonicera similis Hemsl leaves Molecular docking Network pharmacology

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