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Updated: Feb 1, 2026
11:40
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
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Recent Advances in Proximity Labeling-Based Subcellular Proteomic Mapping.
Gang Wang1, Jiapeng Liu2, Xuege Sun3
1College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, PKU-IDG/McGovern Institute for Brain Research, Beijing Advanced Center of RNA Biology (BEACON), Peking University, Beijing, China.
Molecular & Cellular Proteomics : MCP
|January 30, 2026
Summary
New proximity labeling (PL) methods precisely map cellular proteomes, revealing protein localization, modifications, and interactions. Advanced techniques like optoPL and immunoPL offer spatiotemporal control for detailed subcellular proteome analysis.
Area of Science:
- Cellular Biology
- Proteomics
- Biochemistry
Background:
- Cellular proteome spatial organization is crucial for physiological functions.
- Protein localization influences post-translational modifications, trafficking, and interactions.
- Understanding subcellular proteome organization enhances knowledge of protein functions.
Purpose of the Study:
- To review emerging trends in proximity labeling (PL) techniques.
- To highlight advancements in mapping subcellular proteomes with high precision.
- To discuss new PL methods offering enhanced spatiotemporal control.
Main Methods:
- Enzyme-mediated proximity labeling (PL) techniques (e.g., TurboID, APEX2).
- Review of emerging PL methods for subcellular proteome mapping.
- Discussion of photoactivatable PL (optoPL) and antibody-targeted PL (immunoPL).
Main Results:
- PL techniques enable precise mapping of subcellular proteomes in living cells.
- Emerging PL methods provide multi-dimensional protein features (PTMs, trafficking, turnover, interactions).
- optoPL and immunoPL offer enhanced spatiotemporal control and detailed mapping without genetic manipulation.
Conclusions:
- Advanced PL techniques are revolutionizing subcellular proteome analysis.
- These methods provide unprecedented detail on protein localization and function.
- Future research can leverage these tools for comprehensive proteome mapping.
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