Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Isothermal Processes01:21

Isothermal Processes

5.0K
A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes.
An ideal gas can also undergo isothermal expansion or compression.
For example, consider 1 mole of an ideal gas inside an isolated cylinder at initial volume V...
5.0K
Feedback Loops01:01

Feedback Loops

64.4K
In most cases, excessive hormone production is prevented by negative feedback—a loop that starts with a stimulus inducing the release of a particular substance, like a hormone, to maintain a certain level before triggering a signal that results in a decrease in further release of the hormone.
64.4K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

7.3K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.3K
Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

110.9K
Overview
110.9K
Open and closed-loop control systems01:17

Open and closed-loop control systems

1.7K
Control systems are foundational elements in automation and engineering. They are broadly categorized into open-loop and closed-loop systems. These classifications hinge on the presence or absence of feedback mechanisms, significantly influencing the system's performance, complexity, and application.
An open-loop control system operates without feedback from the output. It consists of two primary elements: the controller and the controlled process. The controller receives an input signal...
1.7K
Positive and Negative Feedback Loops01:18

Positive and Negative Feedback Loops

25.2K
Animal organs and organ systems constantly adjust to internal and external changes through a process called homeostasis ("steady state"). Examples of these changes include regulation of the level of glucose or calcium in the blood or internal responses to external temperatures. Homeostasis requires  maintaining an internal dynamic equilibrium:
25.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distribution of virulence genes in clinical isolates of hospital-associated and community-associated methicillin-susceptible Staphylococcus aureus from Terengganu, Malaysia.

Tropical biomedicine·2024
Same author

In silico structural modeling and quality assessment of Plasmodium knowlesi apical membrane antigen 1 using comparative protein models.

Tropical biomedicine·2022
Same author

A conventional multiplex PCR for the detection of four common soil-transmitted nematodes in human feces: development and validation.

Tropical biomedicine·2022
Same author

Bioinformatics characterization of Plasmodium knowlesi apical membrane antigen 1 (PkAMA1) for multi-epitope vaccine design.

Tropical biomedicine·2021
Same author

Toxoplasma gondii infection among selected indigenous community in Sarawak, East Malaysia.

Tropical biomedicine·2021
Same author

Genetic diversity of Merozoite Surface Protein-1 gene block 2 allelic types in Plasmodium falciparum isolates from Malaysia and Thailand.

Tropical biomedicine·2021

Related Experiment Video

Updated: Feb 2, 2026

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.3K

Colorimetric tetra-combo loop-mediated isothermal amplification assay for efficient SARS-CoV-2 detection.

M S Chow1, B P Kee1, P C Lee2

  • 1Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, 50603, Malaysia.

Tropical Biomedicine
|January 31, 2026
PubMed
Summary

A new tetra-combo LAMP assay offers rapid and accurate detection of SARS-CoV-2, targeting four genes. This method provides an efficient alternative for mass and on-site screening of COVID-19, crucial for ongoing pandemic management.

More Related Videos

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
03:53

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Published on: November 10, 2023

2.0K
Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
07:13

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

4.7K

Related Experiment Videos

Last Updated: Feb 2, 2026

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.3K
Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
03:53

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Published on: November 10, 2023

2.0K
Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
07:13

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

4.7K

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • The COVID-19 pandemic, caused by SARS-CoV-2, necessitated global control measures like vaccination and social distancing.
  • Prolonged non-pharmaceutical interventions negatively impacted economies, and emerging SARS-CoV-2 variants challenge vaccine efficacy.
  • Efficient, scalable diagnostic methods are essential for managing COVID-19 and preventing widespread outbreaks.

Purpose of the Study:

  • To develop an efficient detection assay for SARS-CoV-2 suitable for mass and on-site screening.
  • To create a rapid diagnostic tool to aid in the ongoing management of the COVID-19 pandemic.

Main Methods:

  • Development of a tetra-combo Loop-mediated Isothermal Amplification (LAMP) assay targeting four SARS-CoV-2 genes (RdRp, S, E, N) and an internal control (RNaseP).
  • Validation of the LAMP assay using 370 RNA samples from nasopharyngeal and/or oropharyngeal swabs.
  • Performance comparison with the gold standard quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay.

Main Results:

  • The tetra-combo LAMP assay demonstrated comparable performance to qRT-PCR.
  • Achieved a high sensitivity of 98.13% and specificity of 100% for samples with Ct values <= 35.
  • Provided a significantly shorter turnaround time of 25 minutes compared to qRT-PCR.

Conclusions:

  • The developed tetra-combo LAMP assay is a highly sensitive and specific method for SARS-CoV-2 detection.
  • Its rapid turnaround time and suitability for mass screening make it a valuable tool for COVID-19 management.
  • This assay offers an efficient alternative for timely diagnosis and control of SARS-CoV-2 transmission.