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Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
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Colorimetric tetra-combo loop-mediated isothermal amplification assay for efficient SARS-CoV-2 detection

M S Chow1, B P Kee1, P C Lee2

  • 1Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, 50603, Malaysia.

Tropical Biomedicine
|January 31, 2026
PubMed

Insights

A new tetra-combo LAMP assay offers rapid and accurate detection of SARS-CoV-2, targeting four genes. This method provides an efficient alternative for mass and on-site screening of COVID-19, crucial for ongoing pandemic management.

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • The COVID-19 pandemic, caused by SARS-CoV-2, necessitated global control measures like vaccination and social distancing.
  • Prolonged non-pharmaceutical interventions negatively impacted economies, and emerging SARS-CoV-2 variants challenge vaccine efficacy.
  • Efficient, scalable diagnostic methods are essential for managing COVID-19 and preventing widespread outbreaks.

Purpose of the Study:

  • To develop an efficient detection assay for SARS-CoV-2 suitable for mass and on-site screening.
  • To create a rapid diagnostic tool to aid in the ongoing management of the COVID-19 pandemic.

Main Methods:

  • Development of a tetra-combo Loop-mediated Isothermal Amplification (LAMP) assay targeting four SARS-CoV-2 genes (RdRp, S, E, N) and an internal control (RNaseP).
  • Validation of the LAMP assay using 370 RNA samples from nasopharyngeal and/or oropharyngeal swabs.
  • Performance comparison with the gold standard quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay.

Main Results:

  • The tetra-combo LAMP assay demonstrated comparable performance to qRT-PCR.
  • Achieved a high sensitivity of 98.13% and specificity of 100% for samples with Ct values <= 35.
  • Provided a significantly shorter turnaround time of 25 minutes compared to qRT-PCR.

Conclusions:

  • The developed tetra-combo LAMP assay is a highly sensitive and specific method for SARS-CoV-2 detection.
  • Its rapid turnaround time and suitability for mass screening make it a valuable tool for COVID-19 management.
  • This assay offers an efficient alternative for timely diagnosis and control of SARS-CoV-2 transmission.

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