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Updated: Feb 2, 2026

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
Published on: February 27, 2019
Generation and functional evaluation of STAb-T cells
Anaïs Jiménez-Reinoso1, Belén Blanco1, Luis Álvarez-Vallina1
1Cancer Immunotherapy Unit (UNICA), Department of Immunology, Hospital Universitario 12 de Octubre, Madrid, Spain; Immuno-Oncology and Immunotherapy Group, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Madrid, Spain; H12O-CNIO Cancer Immunotherapy Clinical Research Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
Abstract:
STAb-T therapy is an emerging cancer immunotherapy strategy that combines the advantages of adoptive cell therapies and bispecific antibodies. STAb (Secreting T cell engager bispecific Antibodies) T cells are T lymphocytes genetically engineered to secrete bispecific T cell engagers (TCEs) that recruit T cells to target and destroy tumor cells. Adoptive transfer of STAb-T cells has demonstrated potent anti-tumor activity in preclinical models and evaluation in human patients is forthcoming. Here, we provide comprehensive guidelines for generating STAb-T cells and assessing their functionality and efficacy in vitro and in relevant in vivo models of hematological malignancies.
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