In vitro generation of human CD14⁺ MDSC-like cells from normal human monocytes

Myeloid-derived suppressor cells (MDSC) are heterogenous group of immature and mature myeloid cells that accumulate in chronic inflammatory conditions and contribute to the immune suppression. Their ability to inhibit immune responses makes them critical targets for therapeutic interventions. However, working with patient-derived MDSC poses challenges. Variability in cell yields and the complexity of their isolation procedures significantly hinder the conduct of in vitro experiments aimed at investigating potential targeting strategies. In this chapter, we introduce a method to generate human CD14⁺ MDSC-like cells in vitro from healthy donor monocytes. This method provides a consistent and reproducible framework for studying MDSC functions and testing potential inhibitors of their activity.