Framework Doping Enhances COF-DNA Nanoplatforms for Dual RNA Imaging and Phototherapy
Peng Gao1, Mengyao Yu1, Ruyue Wei1
1College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.
Abstract:
DNA-engineered covalent organic frameworks (COFs) hold great promise for cancer theranostics. However, regulating the interaction between COFs and functional DNAs remains a significant challenge, despite its crucial role in ensuring the theranostic performance. In this study, we present a framework doping strategy that simultaneously optimizes the diagnostic imaging and phototherapeutic effects of the COF-DNA nanosystems. In situ doping of porphyrin allows the photocatalytic generation of reactive oxygen species (ROS) by COF nanoparticles and facilitates the adsorption and quenching of fluorescent single-stranded DNA (ssDNA). This enabled the construction of a high-performance COF-DNA nanosystem for RNA-imaging guided phototherapy: the system restored intense fluorescence signals in response to cancer-associated mRNA and miRNA and generated abundant ROS upon laser irradiation, thereby simultaneously lighting up and killing cancer cells. The developed framework doping strategy provides valuable insights into regulating COF-DNA interactions and constructing high-performance theranostic nanosystems.
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