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Updated: Feb 3, 2026

A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
Published on: December 28, 2021
Structure-optimized Au@Pt nanozymes enable robust dual-signal ELISA for on-site screening of ractopamine in swine
Boyan Sun1, Linqian Qin1, Vasily G Panferov2
1State Key Laboratory of Veterinary Public Health and Safety, Key Laboratory for Detection of Veterinary Drug Residues and Illegal Additives of Ministry of Agriculture and Rural Affairs, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Abstract:
Ractopamine (RAC), a banned β-adrenergic agonist, poses severe risks to human health, necessitating rapid and ultrasensitive detection methods. This work presents a novel dual-mode immunoassay utilizing peroxidase-mimicking Au@Pt core-shell nanozymes for the colorimetric and photothermal detection of RAC. The optimized one-step synthesized Au@Pt nanoparticles (Au/Pt = 1:1) exhibited exceptional peroxidase-like activity (27.32 U/mg). After conjugation with monoclonal antibodies, the nanozymes served as immunoprobes in a one-step enzyme-linked immunosorbent assay (ELISA). The enhanced catalysis was elucidated by reactive oxygen species trapping, confirming hydroxyl radicals and singlet oxygen as key intermediates. The assay demonstrated outstanding sensitivity, with limits of detection at 5.32 pg/mL (colorimetric) and 1.03 pg/mL (photothermal), a significant improvement over conventional ELISA. Practical applicability was confirmed in spiked pork and pig urine, achieving recoveries of 86.0-113.0% (coefficient of variation<14.3%), offering a robust platform for on-site screening.
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